The Beckman Center for Cryo-EM is seeking a Sr. Research Specialist II who is an expert electron microscopist to support the center’s operations in cryo-electron tomography (cryo-ET), focusing on lamella preparation and cryo-correlation with focused-ion beam scanning electron microscopy (FIB-SEM) and cryo-correlative light electron microscopy (Cryo-CLEM). The Beckman Center houses state-of-the-art instrumentation and support infrastructure including the Titan Krios equipped with a Falcon 4i direct electron detector and Selectris energy filter, a Glacios equipped with a Falcon 4i direct electron detector, and an Aquilos II cryo-CLEM/FIB-SEM with iFLM. The Sr. Research Specialist II uses specialized scientific knowledge and experience to make decisions, trains/guides other laboratory personnel, and provides simple to complex troubleshooting.

Specific Duties & Responsibilities

• Training and assisting users with grid preparation, FIB-SEM, and cryo-CLEM for 3D cryo-ET analysis (primarily utilizing the Aquilos II and associated equipment).
• Day-to-day instrument operation, including FIB milling of cells and tissue, image acquisition, and microscope maintenance.
• Providing user support for image processing and tomographic analysis.
• Maintain high-throughput software pipelines.
• Develop, design and manage specialized projects and/or instrumentation, and coordinates own objectives.
• Utilize specialized knowledge to set up and run sophisticated instrumentation and interpret results.
• Act as consultant to refine or adapt methodologies to fit research objectives.
• Collaborate in development of new protocols and uses of equipment.
• Advise investigators on experimental design and research methods.
• Co-author/author publications.
• Contribute original thoughts, hypotheses, and analysis to write reports, abstracts and manuscripts.
• Provide significant input to research design and data collection and interpretation based on advanced knowledge/ understanding of activity.
• Independently perform research assays specific to the research laboratory interests.
• Collaborate with other lab personnel, managers, and faculty investigators.
• Independently develop protocol and criteria.
• Make decisions regarding research projects that may require assessment, revision of objectives, or altering scientific direction.
• Consider resources and laboratory priorities when making decision.
• Other duties as assigned.

• Significant hands-on FIB-SEM experience, experience in cryo-electron microscopy (cryo-ET / cryo-EM), image processing, and structure modeling or comparable experience and expertise with the relevant techniques.

• The successful candidate must have strong interpersonal communication skills and be able to effectively work with faculty, staff, postdoctoral fellows, and students from a variety of scientific and cultural backgrounds.

Technical Skills & Expected Level of Proficiency

• Data Management and Analysis - Developing
• Experimental Design and Execution - Developing 
• Laboratory Troubleshooting and Technical Problem Solving - Developing
• Project Management - Developing
• Protocol Development and Optimization - Developing
• Scientific Interpretation and Consultation - Developing
• Scientific Writing - Developing

The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs.

• Bachelor's Degree in Biology, Chemistry or related field.
• Five years of experience in a comparable laboratory performing relevant techniques.
• Additional education may substitute for required experience and additional related experience may substitute for required education permitted by the JHU equivalency formula beyond a high school diploma/graduation equivalent, to the extent

• Ph.D. Degree in a relevant field
• Master's Degree with related graduate research.

Classified Title: Sr. Research Specialist II  
Role/Level/Range: ACRP/04/ME  
Starting Salary Range: $64,600 - $113,300 Annually ($89,301 targeted; Commensurate w/exp.) 
Employee group: Full Time 
Schedule: Monday-Friday, 8:30am-5pm 
FLSA Status: Exempt 
Location: School of Medicine Campus  
Department name: SOM Biophy Biophys and Biophys Chemistry   
Personnel area: School of Medicine 

Logo

About Johns Hopkins University

The Johns Hopkins University (JHU) was founded in 1876 as the nation's first research university, dedicated to bringing the benefits of discovery to the world. JHU is the largest private employer in Baltimore and Maryland, making a large economic impact in the city and state. We enroll more than 30,000 full- and part-time students across ten academic divisions, offering in-person and remote learning in over 400 programs. Not only are we located in Baltimore, but we also have a presence in Washington, D.C.

Connections working at Johns Hopkins University

More Jobs from This Employer

https://main.hercjobs.org/jobs/22236585/sr-research-specialist-ii-x28-biophysics-x29