The Johns Hopkins University department of Genetic Medicine is seeking a Research Technologist who will be responsible for sample handling, planning and executing molecular genetics tests (i.e., genotyping or sequencing) on large numbers of DNA samples.

Specific Duties & Responsibilities

• Transfer DNA samples into 96-well plate format.
• Receive and prepare Deepwell DNA stock plates and create working DNA plates.
• Determine concentration and volume of DNA samples using various instruments.
• Dilute samples to working concentrations.
• Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
• Create master mixes; Set up polymerase chain reactions; trouble-shoot such reactions.
• Operate and troubleshoot robotic liquid handlers.
• Prepare and complete SNP genotyping assays including, but not limited to, lllumina assays and workflows.
• Prepare samples for targeted capture of specific regions of DNA for sequencing experiments. This includes library preparation and capture techniques.
• Prepare and complete Next-Gen sequencing assays including, but not limited to, lllumina NovaSeq workflows.
• Perform quality control checks of data using various software, including, but not limited to, Genome Studio, Spotfire and other in-house QC metrics reports.
• Maintain timely and accurate laboratory records (electronic and written).
• Collect, organize, interpret and submit experimental data to database and supervisors.
• Perform general lab maintenance duties.
• Perform validation tests of lab reagents.
• Participate in committees that function to improve processes.
• Communicate effectively to supervisor and team members.
• Perform miscellaneous related duties as assigned.

Special Knowledge, Skills, & Abilities

• Basic understanding of molecular biology and genetics.
• Requires the ability to safely operate scientific laboratory equipment.
• Use of special equipment, machines and computer(s): Centrifuges, lllumina Beadlab system (Tecans, iScans, HiScans, TeFlow), Covaris, BioAnalyzer, Nanodrop instruments, lllumina NovaSeq, SpriWorks, thermal cyclers, robotic and manual liquid handlers, common laboratory equipment, Macintosh, PC computers, UNIX servers, Oracle database.
• The job requires superior organizational skills for time and data management. Ability to function well in a team production setting is necessary.
• Excellent communication (spoken word) skills.
• Position will require flexibility to work hours beginning as early as 6:00 a.m. and/or ending as late as 6:00 p.m.

• Bachelor's Degree in Biology, Chemistry or related field.
• Some related post-undergraduate work experience preferred.
• Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.

Classified Title: Research Technologist 
Role/Level/Range: ACRO40/E/02/CC  
Starting Salary Range: $15.70 - $26.25 HRLY ($43,160 targeted; Commensurate w/exp.) 
Employee group: Full Time 
Schedule: 8.5 hours between hours of 6 AM ? 6 PM Monday-Fri 
FLSA Status: Non-Exempt 
Location: School of Medicine Campus 
Department name: ???????SOM Genetics Ctr for Inhrtd Dis Res CIDR  
Personnel area: School of Medicine 

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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