Research - Laboratory/Non-Laboratory, Staff/Administrative
The Chorba Lab seeks a highly motivated Staff Research Associate I (SRA I) to work as a research technician and laboratory administrator. Under direct supervision and training, the SRA I will work on several highly collaborative projects including 1) the role and druggability of the processing and trafficking pathways of PCSK9 in regulating the LDL receptor, 2) the characterization of CSDE1 as a regulator of LDLR mRNA decay, and 3) the development of methods to chemically perturb protein translation in a sequence specific manner. The SRA I will have many duties relevant to these goals, including the generation of DNA constructs, generation and maintenance of cell lines, and production and purification of recombinant proteins, in addition to standard cell culture and molecular biology techniques. Responsibilities also include assisting with all routine laboratory maintenance (restocking of supplies, assisting with monitoring freezers, and additional duties as necessary). Notably, the successful candidate may be required to attend to cell culture samples or experimental procedures at off-hours or on weekends when necessary.
The SRA I will be involved in analyzing the effects of chemical and biochemical perturbations of specific proteins both in vitro and in cells. This work will require a number of biochemical and biologic techniques including (but not limited to): PCR; DNA and protein gel electrophoresis; Western blotting; standard cell culture; flow cytometry; transfections; DNA/RNA extractions; protein purification; buffer preparation; and microplate assays.
Our research efforts are focused on identifying and validating drug targets and developing novel therapeutic strategies to treat cardiovascular disease, with a particular focus on regulators of lipid metabolism and cholesterol levels. To this end, we pursue the molecular mechanisms of how key players in these pathways interact, and how their dysregulation leads to disease. Our work bridges the interface of chemistry and biology, where we develop cutting-edge chemical, biochemical, and genetic tools to pursue our inquiries. We are an inclusive environment and are not afraid to think big.
Internal Number: 3148715
About University of California, San Francisco
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.