Research - Laboratory/Non-Laboratory, Staff/Administrative
The Washington University Center for Cellular Imaging (WUCCI) at Washington University School of Medicine in St. Louis is a dynamic microscopy center whose mission is to provide reliable and affordable access to state-of-the-art imaging technologies and work collaboratively with Wash U researchers to advance our understanding of human health and disease. The center houses a broad array of optical, electron/cryo-electron, ion and x-ray microscopes as well as support facilities for tissue culture and sample preparation. The Center supports Wash U principal investigators by providing professional guidance in experimental design, sample preparation and data analysis, as well as working to develop and apply new imaging technologies to problems of critical biological significance.
The Research Specialist (Cryo-Electron Microscopy & Structural Biology) will assist in conducting research projects with a specific focus on (1) the generation of single particle and tomographic cryoEM datasets and (2) the application of data processing methods to those datasets to produce structural models of proteins and macromolecular complexes.
This position provides critically complex research and experimentation for department/s allowing the advancement of scientific analysis and discovery. Serves as a subject matter expert offering support, training and guidance relative to the discipline/area. May participate and or present on the research developed, or the established work of those within the department.
Primary Duties & Responsibilities
Performs complex analyses, experiments and project work under general direction of the PI. Serves as representative for PI and ensures procedures and protocols are adhered to consistently and effectively meet departmentâ™s standard. Exhibits a high degree of independent problem solving and ability to conduct research on assigned projects. May serve in lead role, as needed. Tasks may include (1) preparation of biological samples for cryoEM analysis; (2) screening of samples for quality; (3) organizing collection of single particle and tomographic cryoEM data (4) evaluation of data quality and (5) analysis of cryoEM data using open source tools, such as RELION, cisTEM, cryoSPARC etc.
Plans, develops, organizes and formalizes research results and findings. Prepares publication quality micrographs or images/movies for and/or present on behalf of department. Conducts literature searches related to research projects. Writes/co-authors publications and/or papers supporting research findings in logical and sometimes multiple formats tailored to audiences. May participate in work related to grants and progress reports to funding agencies.
Solves practical problems relating to difficulty with equipment. Suggests technical/procedural improvements in various methods. May develop and optimize experimental procedures and protocols. Provides high-level troubleshooting to advance process or lab procedures. Liaises with instrument manufacturers to schedule preventative maintenance. Maintains ancillary cryoEM equipment. Performs alignment of microscopes prior to data acquisition runs.
Serves as the departmentâ™s research representative and may participate in various related meetings and events. Gathers/delivers data/information and serves as subject matter expert. Performs data analysis on single-particle and tomographic cryoEM datasets. Interfaces with RIS team to coordinate Centers use of institutional storage and HPC resources. Presents research information to internal and/or external audiences.
Provides training to cryoEM facility users by explanation of theoretical principles and practical demonstration of equipment. Provides advanced-level instruction on an individual level with undergraduate students, graduate students, post-docs, and collaborators on the appropriate methods to vitrify samples (both for single particle and tomography). Develops on-line text and video-based training tools for users and lab staff. Provide users with assistance and troubleshooting help as required.
Organizes and maintains lab notebook containing required documentation on lab and/or specimen conditions and can develop and summarize information into usable formats or presentations on behalf of the department. May communicate findings to various users or external audiences.
Performs all related work in accordance with established protocols and safety/compliance requirements. Ensures assigned areas, and various pieces of equipment are always kept clean and prepared. Ensures Center is in compliance with EH&S protocols and procedures. Maintains records of equipment usage and report maintenance issues.
Theoretical and practical knowledge of tissue structure on an anatomical level.
Ability to contribute to experimental design and assist with the analysis and interpretation of results.
Proven organizational and time management skills to successfully set priorities, meet established deadlines and recognize new problems with constantly changing priorities and frequent interruptions.
Effective verbal, written and interpersonal communication skills with the ability to present on various research topics to internal or external audiences.
Ability to diplomatically and professionally interact with all levels of University faculty and staff and external contacts.
Self-motivated and ability to work effectively in a dynamic team environment.
Ability to maintain complete and organized records and reports.
Demonstrated ability as a self-starter with strong critical thinking and attention to detail.
Demonstrated ability and knowledge of computer software & lab equipment, including statistical software, spreadsheets, & lab management systems.
Experience in working in a Linux/UNIX environment.
Experience with cluster computing is desired.
Ability to work in lab research environment.
Masterâ™s degree in a related field with seven years of experience in a laboratory setting or an equivalent combination of relevant education and work experience equaling at least ten years.
$38,900.00 - $70,600.00 / AnnuallyThe salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates within the range will be determined by factors including one's qualifications and performance, equity with others in the department, market rates for positions within the same grade and department budget.
All external candidates receiving an offer for employment will be required to submit to pre-employment screening for this position. The screenings will include criminal background check and, as applicable for the position, other background checks, drug screen, an employment and education or licensure/certification verification, physical examination, certain vaccinations and/or governmental registry checks. All offers are contingent upon successful completion of required screening.
Washington University in St. Louis is committed to providing a comprehensive and competitive benefits package to our employees. Benefits eligibility is subject to employment status, full-time equivalent (FTE) workload, and weekly standard hours. Please visit our website at https://hr.wustl.edu/benefits/ to view a summary of benefits.
Washington University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration without regard to an individualâ™s sex, race, color, religion, age, disability status, protected veteran status, national or ethnic origin, gender identity or expression, sexual orientation. Women, minorities, protected veterans and the disabled are strongly encouraged to apply.
Washington University is dedicated to building a diverse community of individuals who are committed to contributing to an inclusive environment â“ fostering respect for all and welcoming individuals from diverse backgrounds, experiences and perspectives. Individuals with a commitment to these values are encouraged to apply.
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