Research - Laboratory/Non-Laboratory, Staff/Administrative
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The UCSD Department of Obstetrics, Gynecology and Reproductive Sciences is composed of 65 faculty and clinical fellows. The department is composed of 6 divisions: Obstetrics and Gynecology, Maternal-Fetal Medicine, Gynecologic Oncology, Reproductive Endocrinology and Infertility, Hospitalist, and Female Pelvic Medicine and Reconstructive Surgery.
Under supervision, the Lab Assistant will assist the PI with research on the effect of pregnancy and maternal birth injury on the female pelvic soft tissues. Under the supervision, the Lab Assistant will help with the studies of pelvic muscle stem cells. Specifically, the lab assistant will help with tissue harvesting and processing for histological and immuno histochemical analyses, including tissue sectioning, immunostaining, imaging, and data analyses. This Lab Assistant will also assist with tissue preparation for flow cytometry and maintenance of cells in culture. The lab assistant will also be involved in maintenance of the rat, including mating laboratory rats and genotyping using established PCR techniques; as well as in vivo animal experiments such as survival surgery, injections, and monitoring rats during gestation. The Lab Assistant will maintain accurate records of all experiments assist with media and buffer preparation, learn and maintain laboratory equipment, have strong organizational skills to maintain lab stock databases, and work to ensure that laboratory spaces are clean, safe and compliant with University regulations. In addition, Lab Assistant will occasionally need to transport biological specimens.
Two years of laboratory experience OR two years of college including courses in physical or biological sciences and one year of laboratory experience, OR an equivalent combination of education and experience.
Familiarity with small animal handling and managing breeding colonies. Ability and willingness to handle small laboratory animals, assist with survival and terminal procedures, and in vivo injections.
Ability to work with cell culture, flow cytometry, media preparation, tissue histology; assist with genotyping using DNA isolation, PCR-based methods, and gel electrophoresis.
Demonstrated ability to utilize analytical skills, and knowledge to perform basic laboratory calculations.
Ability to train others in various lab techniques and procedures (safety precautions, preparation of media, dishwashing, equipment usage, laboratory maintenance, etc.).
Ability and willingness to follow step-wise experimental protocols.
Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.
Familiar in proper handling of chemical and biohazard materials and sterile techniques. Ability to use basic laboratory equipment including centrifuges, spectrophotometers, balances, pH meters, and electrophoresis equipment.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Proficient in the use of Microsoft Word, Excel, PowerPoint, internet and email with a willingness to learn new programs as needed.
Ability to maintain confidentiality of matters related to research.
Familiarity of purchasing and reimbursement procedures. Skill in maintaining steady levels of lab supplies, and purchasing records, and assist in training others in purchasing procedures.
Knowledge of library usage to locate journals in stacks and from other sources, and the ability to copy specific articles for research.
Theoretical knowledge of cellular and molecular biology, and biochemistry.
Ability to perform molecular biology techniques, including PCR, RT-PCR, cDNA synthesis, gene expression analysis, RNA/DNA isolation.
Familiarity with computer software and databases to analyze data, store results and compute raw data.
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