JOB SUMMARY/ESSENTIAL JOB FUNCTIONS: The main research objective is to describe the role of calcium sensors proteins (STIM1 and Orai) in vascular smooth muscle and the heart. The goal is to define the importance of various calcium signals as mediators in the development of cardiovascular diseases such as, cardiac hypertrophy and hypertension. The successful candidate will be responsible for maintaining cell culture and animal colonies, performing in-vivo (mouse) assays in line with the research strategy and objectives of the lab.
DUTIES AND RESPONSIBILITIES:
Perform laboratory tests: DNA isolation for PCR genotype.
Maintain cell culture and change media.
Assembling Western blot sample preparation for various essays.
Organ harvesting after fixation or snap-freezing.
Isolate primary muscle cells and fibroblast from rat and mice.
Perform routine histology tissue preparation and slides.
Engage in mouse colony maintenance: Mouse weaning, tail-snip, and mouse weaning.
Maintain the animal census.
Manage lab supplies and equipment.
Operate and maintain lab equipment
Performs other duties as required and necessary.
Internal Number: 220616
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