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Application Review Date
The First Review Date for this job is: 6/18/2021
The Wilson lab is dedicated to development of new technologies enabling therapeutic genome editing. To do this, two key resources are essential: highly pure genome-editing enzymes, as well as made-to-order cell lines for use as reporters in genome-editing assays. To this end, we are seeking a skilled researcher capable of producing bespoke proteins and cell lines to support Wilson lab research efforts.
Cloning & purification of made-to-order genome-editing enzyme samples. An example project to be performed with minimal supervision might be clone Cas9 fused to an antibody-tethering domain into an expression vector, recombinantly express the novel construct, and then purify it, developing a novel protocol if necessary. Performing such tasks independently requires expert-level skills including but not limited to: familiarity with current best-in-practice cloning strategies, awareness of structure/function relationships of genome-editing enzymes such as Cas9, and protein engineering best practices such as the impact of incorporating fusion domains and/or codon optimization.
Generation of novel reporter cell lines for genome editing assays. An example project to be executed with minimal supervision might be generate a stably integrated reporter for single-nucleotide changes (i.e. base editing) based HEK293 cells, using an appropriate lentiviral strategy and a suitable cassette design (including antibiotic resistance, etc.). Performing these tasks is only possible with extensive experience in cell engineering as well as moderate experience with genome editing.
Validation & QC of above research assets via techniques such as gel electrophoresis, FPLC-based chromatography, mass spectrometry, immunohistology, flow cytometry, and DNA analysis.
Training of mentees in established research protocols.
Note-taking & data maintenance.
Co-authoring manuscripts to which they contributed.
Other duties as assigned.
Extensive experience with molecular cloning, recombinant protein expression (including knowledge of various helper cell lines & plasmids), and protein purification (including use of various FPLC-based chromatography approaches: affinity, ion exchange, size exclusion).
The ability to troubleshoot techniques and experiments, adapt published protocols to specific experiments, and develop new experimental methods as needed.
Ability to work on multiple assignments, set priorities, reprioritize with ease mid-task, and take initiative in defining and executing work. Ability to work independently as well as collaboratively on a team.
Excellent interpersonal communication skills, especially regarding technical details, instrumentation, etc.
Excellent organization skills (keeping track of work schedule, paperwork, sample storage, and inventory), including use of electronic/shared labiratory notebook systems (e.g. Benchling).
General computer skills (Microsoft Word, PowerPoint, Excel, cloud-based Google apps) for keeping track of results and sharing progress, including use of electronic laboratory inventory systems.
Experience with standard molecular biology techniques: PCR; electrophoresis; western blot; microscopy.
Must adhere to all campus and EH&S safety policies.
At least 3 years of post-graduate research experience.
Experience with cell-line generation including lentiviral production/transduction, single-cell sorting to produce clonal lines, and characterization via immunohistology.
Please submit your cover letter and resume as a single attachment when applying.
Equal Employment Opportunity
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see:
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