The Department of Microbiology & Immunology is seeking a full-time laboratory Technician B to provide technical support for research on bacterial dormancy in the Dworkin Laboratory.
The Dworkin laboratory is interested in the mechanisms used by quiescent cells to survive stressful conditions, such as nutrient limitation with particular attention on how these strategies affect antibiotic sensitivity including the emergence of tolerance (â€œpersistersâ€�). We focus on the down-regulation of protein synthesis, with most recent work examining the nucleotide ppGpp and the protein Hibernation Promoting Factor which induces ribosome dimerization. Our work employs a range of techniques, ranging from genetics and physiology to in vitro biochemistry and single molecule biophysical studies.
Responsibilities will include preparation of growth media and stock solutions, ordering replacement supplies and maintaining laboratory equipment. In addition, the technician B will assist members of the laboratory to perform experiments including genomic DNA isolation, PCR, flow cytometry, and microscopy. The technician B will be required to keep detailed records of all activities.
Bachelor's degree in chemistry, biology or physics or equivalent in education, training and experience.
Ability to set up and conduct experiments or activities according to established protocols
Ability to record and maintain data and activities
Knowledge of related basic laboratory techniques
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 515015
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