Research - Laboratory/Non-Laboratory, Research Support - Laboratory/Non-Laboratory, Staff/Administrative
The Research Assistant I works closely with members of the Next Generation Sequencing Core staff to organize and conduct daily procedures related to operational support of core services and efficient use of core equipment.
General responsibilities include interacting with other members of the core staff, the core director, and Salk Institute researchers submitting samples to the core, handling sample data management and assisting researchers on specific projects.
The position is responsible for, with general instruction on routine work, daily bench work involving generation and maintenance of sequencing libraries and sample quantification & quality control, sequencing, and sequencer operation as well as carrying out workflows for established Next Gen sequencing applications for current application.
(80%) Core Facility Support
Carry out procedures associated with DNA sequencing on the Illumina sequencing platform; including DNA and RNA library preparation, and sample quantification & preparation;
Maintain, troubleshoot, and perform day-to-day work on library preparation.
Analyze current process procedures and provide feedback for their optimization and improvement, document processes, improvements to methods, and troubleshoot assays as needed;
Execute advanced next generation sequencing applications such as genomic DNA, ChIP-seq, RNA-seq, small RNA-seq, single cell RNA-seq, ATAC-seq and custom sequencing libraries.
Perform quality control of samples.
Maintain detailed daily records in a well-kept lab notebook, work carefully to control and optimize protocols.
Assist Core Director and Staff with other associated lab duties as required
(20%) Core Facility Maintenance
Perform routine maintenance and cleaning of core instrumentation
Maintain database of scientific core facility records and adequate inventory of materials.
Perform general maintenance to ensure assigned areas and equipment are maintained
Maintain a clean, orderly work area by dispose of lab waste and chemical waste as appropriate
Prepare buffers and reagents
Reorder of lab supplies and restocking work areas as required
Follow EH&S protocols and procedures to ensure compliance
Keep informed of new developments and technical advances
No professional experience required with relevant Bachelor’s degree or above
Prior hand-on experience with sequencing platforms (including DNA and RNA library preparation, sample quantification & preparation, and sequencer operation);
Demonstrated experience with overseeing general lab maintenance.
Prior experience working in core facility environment
Bachelor’s degree in molecular biology, cell biology, or Genetics or related discipline, or equivalent.
SKILLS AND ABILITIES
Practical and theoretical knowledge of biological sciences (particularly cell and molecular biology and genetics)
Able to modify or adapt standard procedures to meet the needs of specific research projects
Ability to solve challenging problems on a daily basis
Proven organizational and time management skills to successfully set priorities, meet established deadlines, multi-task projects, adjust to changing priorities and recognize new problems with constantly changing priorities and frequent interruptions
Ability to work both independently and as part of a collaborative team
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment; ability to read, comprehend, and discuss research materials
Skills utilizing sound judgment, tact and a recognition of situations requiring confidentiality
Ability to diplomatically and professionally interact with all levels of Institute staff and external contacts
Knowledge of standard laboratory and safety procedures
Demonstrated knowledge and experience with PC and MAC operating systems, standard business office equipment and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.
Knowledge of molecular biology equipment and procedures (including RNA and DNA preparation, RNA and DNA quantification, hybridization, PCR, quantitative PCR)
Knowledge in next generation sequencing
SPECIAL CONDITIONS OF EMPLOYMENT
Must be willing to work in an animal-related research environment.
Must be willing to work an adjusted schedule to support research needs.
Successful completion of the Institute’s background investigation.
This position requires constant adjusting focus, seeing, sitting, touching/feeling, analyzing, calculating, reading, and working inside.
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information. 41 CFR 60-1.35(c)
Salk scientists are dedicated to innovative biological research. The Salk Institute consistently ranks among the leading research institutions in the world for its faculty's contributions and the impact of their findings. Focused both on discovery and on mentoring future generations of researchers, Salk scientists make groundbreaking contributions to our understanding of cancer, aging, Alzheimer's, diabetes and infectious diseases by studying neuroscience, genetics, cell and plant biology, and related disciplines. "We lead biological research. We prize discovery. Salk is where cures begin."Overlooking the Pacific Ocean in La Jolla, the Salk Institute offers a warm, collegial and collaborative work environment, where employees enjoy a generous benefits program. Social activities on campus encourage interaction as a community.