Research - Laboratory/Non-Laboratory, Staff/Administrative
50% - Conducts behavioral experiments with rats and mice, including, but not limited to: performing aseptic stereotaxic surgeries, taking regular food and body weight measurements, measuring spontaneous physical activity (SPA), measuring body composition, measuring energy expenditure, and performing trans-cardiac perfusions with minimal supervision.
40% - Transgenic mouse colony management, including implementation of breeding strategy, PCR screening protocols, employment of methods for controlling genetic drift, regular colony up keep (e.g. breeding, weaning, ear tagging, DNA extraction, and euthanasia) and record keeping, with minimal supervision. The ability to modify colony dynamics to meet dynamic experimental needs of lab members. Immunohistochemical (IHC) analysis of brain tissue, including but not limited to: cryostat sectioning of brain tissue, immunofluorescent (IF) staining and microscopy.
10% - Keeps records of all study details in a lab notebook, to be accessible to all lab members. Presents data and interpretation of findings to the lab group, on a regular basis. Keeps inventory of and places orders for lab supplies, reagents, and animals. Maintains compliance with all regulating bodies (e.g. IACUC, IBC, OSHA). Participates in maintaining lab organization. Maintenance, modification, and calibration of equipment. Leads in purchasing negotiations for equipment and service agreements with vendors.
Required Qualifications (must be mentioned on application and/or resume):
Bachelors degree in Biology, Physiology, Neuroscience or related field and one year of professional experience or a combination or education and experience to equal 5 years.
Experience with stereotaxic surgeries and handling viral vectors.
Experience with immunohistochemical techniques and fluorescence microscopy.
Demonstrated experience working with animals, including colony maintenance and running behavioral neuroscience experiments with rats and/or mice.
Experience in reverse transcriptase polymerase chain reaction (rtPCR).
Ability to work well within the lab group and with collaborators.
Must have excellent oral and written communication skills.
Willingness to learn new techniques and methods.
Evidence of being capable of troubleshooting experimental problems
Internal Number: 340525
About University of Minnesota, Twin Cities
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