Research - Laboratory/Non-Laboratory, Staff/Administrative
Duties: 60% - Perform complex weekly experiments, analyze results and maintain appropriate records Laboratory techniques may include all of the following: Transduction of primary immune cells for development of a cell therapy product, flow cytometry, real time PCR, immunohistochemistry, in situ tetramer staining, RNAscope, confocal and light microscopy, image analysis, Western blotting, ELISA, animal (mouse) handling
Expectations: â—Prepare for and successfully execute experiments with guidance from their supervisor â—Analyze data from experiments and present in appropriate format at lab meetings, in meetings with collaborators, and for grants, publications and conferences â—Research relevant literature to keep up-to-date with advances in scientific knowledge â—Follow existing laboratory protocols and develop new protocols as needed â—Function as a member of a team in executing large studies â—Maintain a laboratory notebook â—Train colleagues with less experience in laboratory protocols
25% - Maintenance and production of stocks and reagents for the laboratory Maintenance and production of stocks and reagents for the laboratory Culturing and maintaining cell stocks, preparation of plasmid DNA from bacterial cultures, genomic DNA and mRNA isolation, preparation of retrovirus or lentivirus for cell therapy, isolation of primary immune cells Expectations: â—Production of high quality reagents for the use of other researchers in the laboratory â—Careful documentation of quality control tests of the reagents â—Maintenance of a database of the laboratory produced reagents
15% - General laboratory maintenance â—Maintain laboratory equipment â—Order and maintain laboratory supplies including obtaining quotes from vendors â—Maintain laboratory databases â—Miscellaneous duties as assigned
All required qualifications must be documented on application material
Required Qualifications: BA/BS or a combination or related education and work experience to total 4 years. Experience working with tissues and cells from animals infected with viruses. Proficiency in flow cytometry, real-time PCR, RNAScope, confocal microscopy, and primary lymphocyte cell culture. Proficiency with Fluoview, Photoshop, Prism and FlowJo software.
Preferred Qualifications: Prefer a bachelor’s degree in the biological sciences with a working knowledge of immunology. Prefer previous work with SIV-infected cells and/or tissues Looking for demonstrated ability to carry out precise work in a research laboratory.
Internal Number: 339679
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.