A Post-Doctoral Associate position is available in the new laboratory of Dr. Alon Herschhorn at the Division of Infectious Diseases and International Medicine, Department of Medicine, University of Minnesota.
We are a new and energetic research group that works on exciting directions to understand and target HIV-1 host interactions at the molecular and cellular levels. We use interdisciplinary approaches at the interface of synthetic and molecular virology, immunology and cell biology to gain new insights into complex biological processes, with the aim of translating these insights into novel therapies and vaccines to treat and prevent viral infections.
70% Research Primary duties and responsibilities will include, but are not limited to, studies of virus-host interactions using HIV-1 as a system model. The project involves developing novel viral immunogens and display systems and testing them in animal models, analyzing the antibody and cellular immune response to immunization, studying HIV-1 interactions with neutralizing and non-neutralizing antibodies using viral assays, and building new vectors and genetic circuits to investigate molecular interactions between viruses and the host.
The successful candidate will work in BSL2 and BSL2+ environment and build and use viral vectors, express and purify proteins and viruses, and study the interactions of viruses with the immune system. The work also involves interaction with other lab member(s), participation in lab meeting, and oversee the work of undergraduate students.
30% Data Analysis, Presentations, and Manuscript Presentation Analysis of experimental results.
The successful candidate will use different computer programs and statistical tests to analyze the immune response in animals. Analysis of flow cytometry results will be done using FlowJo or Cyflogic programs and involves analysis of fluorescence readouts, live/dead cells discrimination and multi color compensation. Development of research directions according to the main project aims and the ongoing experiments Preparation of figures and manuscripts based on the results Reading literature related to the research projects and developing collaborations with other research groups Preparation of data for presentations.
Required Qualifications: (Must be documented in application materials)
A Ph.D. (or equivalent) degree in virology, microbiology, cell biology, immunology or a related field
Advanced knowledge in molecular and cellular biology techniques. Experience in immunological methods such as ELISA, antibody characterization, and western blot; high proficiency in PCR amplification, cloning, protein expression and purification, and tissue culture techniques.
Excellent written and verbal communication and organization skills with proficient scientific record keeping
Proficiency in Microsoft Office applications including Word, Excel, and Power Point
Demonstrated ability to interact with other group members and oversee undergraduate work
Ability to work independently, pay attention to details, solve problems, work under pressure and manage multiple tasks
Experience and ability to analyze scientific data, present the research results and prepare manuscripts for publication
Enthusiastic to work in new lab studying virus host interactions; team player, proactive, open-minded, creative and willing to learn
Experience in work with bacteria, human cells/tissues and viruses, including HIV-1, in a BL2+/BSL3 facility
Previous work with HIV-1 assays.
Experience with writing and following an institutional animal care and use committee (IACUC) protocols
Experience with protein expression and purification, and virus-like-particles production and concentration
Advance skills in molecular biology methods, including cloning and PCR, tissue culture work with different cell lines, and transfection.
Experience with isolation of Peripheral Blood Mononuclear Cells (PBMC), NK cells and lymphocytes from blood, and analyzing cytotoxic T cells activity and specificity.
Experience with flow cytometry techniques and multi-parameter analysis
Experience with bioengeening of viral vectors and building new genetic circuits.
Previous work with humanized mouse.
Internal Number: 339586
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.