Research - Laboratory/Non-Laboratory, Research Support - Laboratory/Non-Laboratory, Staff/Administrative
The purpose of the position is to provide technical and experimental support for ongoing research projects in the Molecular and Cell Biology laboratory supporting Dr. Gerald Shadel. The focus of this role will be of mitochondria and mtDNA in diseases, aging and the immune system. The position requires prior laboratory experience and proficient technical skills in molecular and cellular biology, genetics and biochemistry to assist in the execution of specific assays and design and troubleshooting of new experiments and protocols.
Routine specific activities in the position will include mammalian cell culture and transfections for gene knockdown/overexpression; isolation of proteins and nucleic acids from cells and tissues for western blot, quantitative PCR and RNAseq analysis; fluorescence microscopy and other cell imaging modalities; subcellular fractionation; and various assays of mitochondrial function and stress. Incumbent must be able to interact productively with faculty, postdoctoral scholars, graduate students and other lab personnel, as well as capable of working largely independently with only minimal general supervision.
(50%) Molecular Biology Techniques
Protein purification, western blotting, gel electrophoresis.
Perform DNA and RNA assays (RT-PCR, ddPCR, RNAseq analysis).
Perform general molecular biology techniques (plasmid construction/molecular cloning).
(50%) Tissue Culture
Culturing mammalian cell types including primary and cancer cell lines.
Production of lentiviruses.
Perform transfections and lentiviral infection for overexpression/knockdown in various cell types.
Isolation of protein, DNA and RNA from cells and tissue.
Minimum 4 years of experience in molecular or cell biology including experience with mammalian cell culture, and cellular and molecular biology techniques.
Experience with flow cytometry, fluorescence microscopy, and mouse husbandry
Bachelor’s degree of science in Biology/Biochemistry or related field
SKILLS AND ABILITIES
Strong theoretical and practical knowledge of biochemistry, molecular biology, and genetics and associated experimental techniques.
Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions.
Excellent organizational skills including the ability to keep clear and accurate records, organize a varied workload, follow written and oral protocols and give oral presentations on the progress of research in lab meetings.
Ability to prioritize and schedule experiments and duties to maximize efficiency.
Analytical and critical-thinking skills to identify and communicate problems.
Ability to work both independently and as part of a collaborative team.
Strong interpersonal skills, including tact, diplomacy, and flexibility.
Ability to make independent decisions, analyze situations, and solve problems.
Ability to establish and maintain effective working relationships; interact with all levels of personal and management.
SPECIAL CONDITIONS OF EMPLOYMENT
Must be willing to work in an animal-related research environment.
Must be willing to work an adjusted schedule to support research needs.
Successful completion of the Institute’s background investigation.
Must be willing to sign an employee confidentiality agreement.
This position will constantly be adjusting focus, grasping, hearing, keying, seeing, talking, analyzing, calculating, communicating, reading, reasoning, writing and working inside.
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information. 41 CFR 60-1.35(c)
Salk scientists are dedicated to innovative biological research. The Salk Institute consistently ranks among the leading research institutions in the world for its faculty's contributions and the impact of their findings. Focused both on discovery and on mentoring future generations of researchers, Salk scientists make groundbreaking contributions to our understanding of cancer, aging, Alzheimer's, diabetes and infectious diseases by studying neuroscience, genetics, cell and plant biology, and related disciplines. "We lead biological research. We prize discovery. Salk is where cures begin."Overlooking the Pacific Ocean in La Jolla, the Salk Institute offers a warm, collegial and collaborative work environment, where employees enjoy a generous benefits program. Social activities on campus encourage interaction as a community.