Research Support - Laboratory/Non-Laboratory, Staff/Administrative
Rutgers, The State University of New Jersey is seeking a Laboratory Assistant for the Rutgers Institute for Translational Medicine and Science.
The Laboratory Assistant will perform a broad range of experiments related to evaluating cellular responses generated by glucocorticoids and cAMP-elevating agents, encompassing cell and molecular biology, tissue culture and animal work techniques. Primary responsibilities will include culturing different lung cell types (both primary cells and cell lines), performing human and murine Precision Cut Lung Slice (PCLS) experiments, performing immunological assays (immunofluorescent staining, ELISA, western blots, immunoprecipitations, and flow cytometry/FACS), and molecular biology techniques (PCR & real time-PCR techniques, DNA and mRNA sample preparation, plasmid DNA preparation, DNA subcloning, and siRNA/shRNA transfections ).
Among the key duties of this position are the following:
Cell Culture work: media preparation, quality control testing, and daily maintenance of human airway muscle cell lines and human fibroblast cell lines.
Protein work: Protein isolation, immuno-precipitations, and western blotting.
Molecular Biology work: RNA/DNA isolation and purification, PCR and real time PCR, transfections, etc.
Maintaining all certifications, laboratory protocols, and experimental write ups.
Minimum Education and Experience:
At least one (1) year of prior laboratory experience.
Required Knowledge, Skills, and Abilities:
Ability to perform basic scientific assays including: RNA/DNA extraction, and protein preparation, PCR and real time-PCR, western blot analysis, ELISA are essential.
Familiarity with basic concepts of cell, cell-cell signaling, and molecular biology.
A background in cell and molecular biology, and tissue culture is a prerequisite for this position.
Strong verbal and written communication skills, ability to work independently and as part of a team.
Associate’s or Bachelor's Degree.
Experience in one of the following fields: biology, cell biology, biochemistry, molecular biology, or other related field.
Candidates with pulmonary research experience.
Physical Demands and Work Environment:
Occasional lifting 25-50 lbs.
Posting Number: 21ST0363
Location: Downtown New Brunswick
Internal Number: 126881
About Rutgers University
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.