JOB SUMMARY/ESSENTIAL JOB FUNCTIONS: Under the direction of an assigned supervisor, the Senior Research Assistant assists with the development of an ongoing research project involving pediatric cardiomyopathies. This position uses animal models to uncover the markers of disease progression at the molecular and physiological levels.
DUTIES AND RESPONSIBILITIES:
Oversees/breeds animal models (transgenic mice) of cardiomyopathy following animal protocol (IACUC).
Assists with tissue collection (blood sampling, tissue extraction), histology techniques, and staining (Eosin & Hematoxylin, picrosirius red, and red Oil O).
Conducts in vivo testing of cardiac function, such as electrocardiogram, echocardiography, and blood pressure measurements.
Enacts molecular biology experimentation, including Western Blot, DNA and RNA extraction, quantitative-PCR, and DNA analysis.
Performs DNA transfection, cell culture, and immunohistochemistry.
Maintains accurate animal log records.
Assists with establishing and maintaining Standard Operating Procedures (SOPs).
Provides routine laboratory maintenance and lab safety checks and maintains lab safety logs.
Orders, tracks, and maintains research supplies and inventory.
Instructs and trains staff members, fellows, and students.
Inputs data and analyses using Excel, GraphPad, and SPSS.
Coordinates equipment use and ensures general maintenance of equipment.
Performs other related duties as assigned.
Internal Number: 211506
The mission of the University of Tennessee Health Science Center is to bring the benefits of the health sciences to the achievement and maintenance of human health, with a focus on the citizens of Tennessee and the region, by pursuing an integrated program of education, research, clinical care, and public service.