The Technician will assist in carrying out and carefully documenting experiments primarily focused on the regulation of T cell responses by macrophages in normal human tissues and the role of these cells in pathogenesis of severe inflammatory reactions within certain vital organs. Disease states currently under investigation include pancreatitis and COVID-19 acute respiratory distress syndrome. Experiments will entail immune cell isolation from human tissues, performing fluorescence activate cell sorting and using the sorted immune cell populations in functional in vitro assays. In addition, the immune cells will be analyzed using high throughput genomic techniques including bulk and single cell RNAseq. The technician will also perform spatial analysis of immune cells in human tissues assisting in quantitative analysis of fluorescence microscopy images from human tissues and participate in spatial transcriptomics studies.
Careful training and oversight by the principal investigator and an experienced research technician will be provided for all aspects of this job. Opportunities for co-authorship on research manuscripts will be provided and the technician ideally would be available for at least a 2-year commitment in order to bring their research projects to completion.
Most work will be conducted during regular business hours however working on the weekends will be required (0-2 times a month depending on need). Weekend responsibilities will be scheduled in advance and shared with other members of the research team.
Responsibilities will include: human immune cell isolation from solid organs as well as blood, flow cytometry panel design, operation of analytical flow cytometer, supervision of fluorescence activated cell sorting, tissue culture experiments including functional experiments on primary human cells, cryopreservation and curation of human tissue samples, distribution of research samples to collaborating investigators and research core facilities, acquisition and analysis of fluorescence images for quantitative spatial analysis.
Conduct experiments (80%)
Organize, preserve and distribute human samples for collaborators (20%)
Bachelor's degree in chemistry, biology or physics or related science or equivalent in education, training and experience
Basic knowledge of immunology from coursework or equivalent research experience. Basic knowledge of scripting languages for data organization and analytics including R and/or Python and willingness to build a skill set for large scale, multidimensional data analysis and systems biology.
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 512708
About Columbia University
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.