Research Support - Laboratory/Non-Laboratory, Staff/Administrative
Staff Research Associate (9612C) - 14539
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Application Review Date
The First Review Date for this job is: January 28, 2021
The Department of Molecular & Cell Biology (MCB) is home to undergraduate and graduate academic programs as well as Faculty Research performed by faculty organized in five divisions:
Biochemistry, Biophysics and Structural Biology
Cell and Developmental Biology
Genetics, Genomics and Development
Immunology and Pathogenesis
The focus of the Hockemeyer Lab is to establish reliable models of human diseases using genetically engineered human and stem cell and mouse models and functional genomics screening approaches. We are particularly interested in developing rigorous genetic approaches to study human disease, development and cell biology where conventional animal models or tissue culture systems do not adequately recapitulate human biology. To do this we are leveraging our expertise in human stem cell techniques and genome editing, humanized mouse models to elucidate processes of human stem cells renewal and differentiation and during tumor formation. For more information, visit the Hockemeyer Lab website.
Isolation and generation of mouse embryonic fibroblasts for the lab. This includes weekly thawing, passaging, counting and plating of cells. In addition, this involved to newly isolate MEFs and replenish frozen MEFs stocks. To do this the SRA will learn how to set up breeding's, weaning's, and euthanizing mice.
Assisting other members in the lab with research projects. Specifically, optimizing and testing tissue culture and molecular biology protocols such as differentiation or staining protocols. This will be done with the guidance of the PI and other lab members. This involves planning, designing the experiments discussing the outline of the experiment/ protocol with the PI, conducting the experiments, analyzing results and interpreting data under the supervision of the PI.
Standard molecular biology and tissue culture techniques.
The SRA will assist lab members with the maintenance and characterization of human stem cell cultures and genotyping of stem cells. To do this the SRA will learn to isolate DNA from human stem cells, amplify DNA by PCR and prepare samples for sequence analysis. This genotyping will require daily record keeping in laboratory notebook to record the samples analyzed and the attendance at weekly lab meetings to understand the basic objectives of the research project.
Lab support duties including autoclaving waste and ordering and restocking supplies.
Assisting with protocol renewals and safety inspections.
Experience with sterile tissue culture techniques, PCR, basic lab maintenance duties, excellent organizational skills and attention to detail.
Bachelor's degree in the life sciences, preferably Biology, Molecular and Cell Biology, Biochemistry, 1-year (cumulative) experience working in a biology research lab and/or equivalent experience/training.
Experience with sterile tissue culture and molecular biology techniques.
Mouse handling and experience with molecular biology techniques, knowledge of lab safety procedures, capability to design and conduct experiments and analyze data.
Salary & Benefits
This is an exempt, monthly paid position. Annual salary is commensurate with experience.
For information on the comprehensive benefits package offered by the University visit:
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