Research - Laboratory/Non-Laboratory, Staff/Administrative
Department: MED-Neurological Surgery Salary/Grade: NEX/9
The laboratory of Dr. Derek Wainwright conducts basic, translational, and clinical studies of brain cancer immunobiology and immunotherapy in mouse models and human patients with glioblastoma; the most common and aggressive form of primary brain cancer. Current research in our lab focuses on understanding how the anti-brain tumor immune response induces higher levels of immune suppression in the cancer, how advanced age increases the incidence and decreases the responsiveness to immunotherapy, and how psychosocial modifiers change the anti-brain tumor immune response to favor tumor cell eradication. We use a combination of immunobiology, gerobiology, neurobiology, cancer biology, cell biology, advance molecular biology techniques such as DNA chip technology, Next-Gen Sequencing, Chip-seq analysis, transgenic animal models and patient derived orthotopic brain tumor animal models, as well as advanced in vivo imaging techniques to understand the molecular mechanisms underlying cancer initiation and therapeutic resistance in mice and humans with brain tumors.
The Research Technologist will principally responsible for all in vivo studies in mice. This includes daily observations and weighing of subjects, preparing the surgery cart, prepping the surgery area, preparing and administering anesthesia and other treatments, and overseeing the recovery of mice after treatments and intracranial injections. Responsibilities also encompass breeding and genotyping. The Research Technologist will study the immunological mechanisms that contribute to immunotherapeutic resistance in cancer while utilizing state-of-the-art facilities to conduct cutting-edge research with the intention of publishing the results in pre-eminent peer-reviewed scientific journals such as Cell or Nature. Specific duties include: conducts tests and procedures; sets up and operates equipment; records observations and measurements and collects as well as compiles data; conducts basic animal and experimental research lab work and/or assist in one or more phases of specific projects.
Follows pre-defined protocols, policies, procedure, regulations, Good Clinical and Good Laboratory Practices and conducts standard and repetitive experiments and completes basic trouble-shooting techniques.
Sets-up, operates and maintains standard lab equipment.
Using an entry level of skill, completes the following activities:
DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
Cloning, mapping, and sequencing of DNA
Recombinant virus production and purification
Cell culture, cell isolation
Breeding and screening transgenic mice
Database management of colony
Records test results and may maintain databases.
Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
Maintains inventory of reagents, mice and lab supplies.
Performs other duties as assigned.
HS diploma or equivalent and some basic science courses is required.
Bachelor’s degree in relevant field (cell biology, molecular biology, genetics, biophysics, etc.)
Experience working on brain tumor or translational cancer research.
Experience working in a laboratory environment with mouse models.
Preferred Competencies: (Skills, knowledge, and abilities)
Demonstrated ability to follow detailed instructions and research protocols.
Demonstrated computer proficiency, including spreadsheets and word processing.
Attention to detail, ability to ensure ongoing organization of laboratory environment.
Precise and accurate record keeping, adherence to ethical standards of research.
Ability to work reliably, both independently and in a team environment.
Ability to assist and train other lab members in routine protocols.
Ability to build rapport with faculty and ancillary staff.
Ability to comprehend and critique published scholarly articles.
Familiarity and experience with animal care and tissue culture methods.
Excellent organizational, oral and written communication skills.
High familiarity with use of Microsoft Office programs (Word, Excel, PowerPoint).
Above average planning, organization and time management skills.
Knowledge of and adherence to safety and regulatory compliance.
Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.
Internal Number: 39947
About Northwestern University
Northwestern University is a major private research university with 12 academic divisions located on three campuses in Evanston, Chicago, and Education City in Doha, Qatar. We have approximately 2,500 full-time faculty members, 17,000 graduate and undergraduate students, and over 5,700 full and part-time staff. Northwestern University combines innovative teaching and pioneering research in a highly collaborative environment. It provides students and faculty exceptional opportunities for intellectual, personal and professional growth.