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Job Summary Studies of small molecule DNA repair inhibitors for use in chemotherapy. This project involves hit-to-lead development of a new class of DNA repair inhibitors. Novel chemical compounds will be screened biochemically for potency and specificity and physiologically for synthetic lethality with various tumor models and on target functions. This will constitute a unique training opportunity for introduction into the biotechnology industry.
DNA preparation, Primer design, Cloning, PCR, RT-PCR
Human tissue cell culture-mycoplasma monitoring, maintining cell line collection, freezing, thawing, cataloging cell lines
Transfection and siRNA and shRNA knockdowns
Cell viability assays
Biochemical assays of enzymes that function in DNA repair or replication
Protein expression and purification
Data analysis, statistical analysis
Use of NCBI, PubMed, BLAST, Genbank; Protein Data Base
Project management: Planning, record keeping,
Lab management and ordering
B.S. degree and at least 4 years of experience in quantitative molecular biology and cell biology in an academic research lab or in the pharmaceutical industry
Demonstrated ability to work independently in execution of experiments, analysis of results, and design of follow-up experiments
Experience in project management
General techniques: Handling DNA and RNA, protein and nucleic acid electrophoresis; Western blotting; Immunoprecipitation, extract preparation, quantitative enzyme assays (nuclease, polymerase, helicase, topoisomerase or other kinetic analyses), transfection and transformation; light mcroscopy
Special techniques (previous experience appreciated but training provided where required): site-directed mutagenesis, Immunofluorescence, immunoprecipitation, affinity purification, protein exression and purification, cell vibility assays; techniques for monitoring DNA replication and recombination, non-B DNA structures , and telomeres.
Familiarity with spectrophotometer, scinitillation counter, phosphorimager (Image J, Imagequant), FPLC, plate readers. Gel doc systems, Licor imaging
Fluency in Word, Excel, GraphPad Prism, Powerpoint
PhD or PhD level experience in academia or in the biotechnology industry
A subset of the skills listed above
Background knowledge in the field of DNA repair, replication or recombination appreciated but not required
Background knowledge in cancer research preferred but not required
We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
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The California Institute of Technology (Caltech) is a world-renowned science and engineering research and education institution, where extraordinary faculty and students seek answers to complex questions, discover new knowledge, lead innovation, and transform our future.