Research - Laboratory/Non-Laboratory, Staff/Administrative
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The Department of Genome Sciences (GS) is part of the University of Washington School of Medicine (UW SoM) and is comprised of 30 labs addressing leading edge questions in biology and medicine by developing and applying genetic, genomic and computational approaches taking advantage of genomic information now available for a host of species. GS is making major contributions in the application of genome science to understand the fundamentals of biology and to improve human health and wellbeing. Genome Sciences is a collaborative department supported by very different academic disciplines. We work with a multitude of Program Investigators (PIs) in departments both on the UW campus as well as worldwide. The department has gone through significant expansion since its beginning in 2001 with annual grant awards of $57M in FY20 and 62 teaching and research faculty.
Here at Shendure lab, we work on the development of innovative technologies in genomics. Our main focus includes generating single-cell Hi-C, ATAC, and RNA-seq data, using CRISPR/Cas9 editing system to investigate functional changes in genome, and translating these genomic findings to the clinic.
This is an academic lab and funded by both federal grants and by the Howard Hughes Medical Institute. We are currently seeking a Flow Cytometry/Cell Sorter Scientist for employment in our laboratory at University of Washington in Seattle, WA.
Independently serve as primary operator for cell sorting on a BD FACS Aria II
Independently optimize high-power lasers; tune laser wavelengths to excitation frequencies necessary for novel fluorochromes or techniques; reconfigure bandpass and directional (dichroic) optical filters to appropriately measure cellular fluorescence.
Design new protocols for sorting and optimize sorting parameters along with verification of sorting purities/efficiencies. Understand compensation and the complexities of polychromatic flow cytometry from 6 -15 colors.
Alter PMT and filter orientations to accommodate the use of non-routine or novel fluorochromes used for immunophenotypic analysis of human peripheral blood cells, human and mouse cells, cellular organelles such as mitochondria, nuclei, or exosomes, and fluorescent proteins expressed using viral transgenes, or other fluorescent makers measuring cell cycle or cellular metabolic activities.
Perform routine maintenance on instruments; resolve instrument technical issues; document usage of instruments.
Provide sample preparation, staining, data acquisition and analysis for a). immunophenotypic samples, b). cell cycle samples, including preparation of custom non-commercial reagents, c). Carry out either proof-of-principle and/or routine experiments for users in order to ensure the validity and integrity of the results and the most efficient use of IRP resources.
Provide data analysis support, including export and presentation of graphic files for inclusion into scientific publications.
Independently analyze data with flow cytometry software packages (FloJo, FCS express, MultiCycle, VenturiOne); ensure that software updates are current.
Maintain laboratory records as they pertain to instrument usage and performance, including daily quality control.
Evaluate current/proposed protocols for validity to ensure the validity of the resulting experimental data; conserve reagent and sample resources.
Prepare educational materials for facility users; provide Pub-Med searches for a variety of alternative protocols and flow cytometric approaches to experimental questions.
Maintain and review core records and ensure electronic records of data on the sorters are backed up and maintained.
Maintain research database for laboratory unit; schedule and maintain an accurate tracking system for all activities, including research documentation.
Maintain a safe work environment in accordance with policies/procedures and UW EH&S safe laboratory practices especially when sorting biohazardous materials; ensure others are aware of the potential safety hazards.
Engage in continuing scientific and technical education; participate in flow cytometry forums and discussions.
Observe and maintain inventory levels for laboratory supplies and equipment for the cell sorting; generate purchase requests for needed supplies and equipment.
Other duties are required in support analysis and sorting of users’ samples.
Provide backup support for complex data acquisition on the BD FACS ARIA II, including samples stained with UV-exciting dyes, cell cycle markers and fluorescent tracking/expression proteins; provide software upgrade training on instrument.
Bachelor's degree in Biology, Life Sciences or a related discipline.
Minimum of three years of experience in related field.
Prior experience sorting on a Becton Dickinson FACS Aria II or similar sorter
Knowledge of managing and disposing of hazardous waste.
Strong communications skills, both oral and written.
Excellent analytical, organizational, interpersonal and time management skills.
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.