Research - Laboratory/Non-Laboratory, Staff/Administrative
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The Division of Allergy and Infectious Diseases in the Department of Medicine has an outstanding opportunity for a full-time Research Scientist/Engineer 3.
A position is available in a cellular immunology lab for an experienced scientist to contribute to several funded projects. Located at UW SLU, projects include research on SARS-CoV-2, HSV-1 and HSV-2, HHV-8, varicella zoster virus, syphilis, and cancer caused by MCPyV, a polyomavirus. Several smaller and larger, short and long term projects allow an additional position to be created, adding to a staff of three doctoral-level UW Research Scientists, one post-doc, and several technologists.
Duties and Responsibilities:
Human T cell culture, T cell functional assays such as ELISPOT, flow cytometry including intracellular cytokine cytometry, ELISA, proliferation, and cytotoxicity,
Virus culture and virus titration by plaque assay,
Dendritic cell and EBV-immortalized B cell culture,
Analytical flow cytometry and live cell sorting including data acquisition and analysis software,
PBMC isolation from blood, routine eukaryotic cell line freeze/thaw/passage
Immunomagnetic cell enrichment, and live cell (eGFP etc.) fluorescence microscopy
DNA quantitation by spectrophotometer,
Cell DNA transfection with lipid reagents,
Basic E. coli culture, basic PCR and cloning, plasmid preps, send-out Sanger sequencing.
As a UW employee, you will enjoy generous benefits and work/life programs. For detailed information on Benefits for this position, click here.
Master's Degree in a science related field and one years' applicable experience to include:
Ability to multitask
Work with internal and external collaborating teams
Prepare concise written reports and graphical output
Maintain Deliverable timelines important.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
Master's degree and three years experience to include:
Facility in basic mouse immunology procedures and T cell assays,
Mouse implantable tumor model experience,
Luciferase based in vitro and in vivo imaging,
Human T cell cloning,
Tissue digestion for leukocyte recovery,
Eukaryotic cell electroporation,
BSL-3 virology experience,
Lentivirus use (expression construct cloning, manufacture via transfection/concentration/titration),
Uploading data to Genbank and similar respositories,
NGS library preparation and data pipelines, and bioinformatics experience with highly dimensional RNAseq, TCRseq, and flow cytometry data.
CONDITIONS OF EMPLOYMENT
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Workforce Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
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