Research - Laboratory/Non-Laboratory, Staff/Administrative
The Department of Medicine, Division of Gastroenterology is seeking a Research Technologist to work under the general supervision of a faculty investigator, the individual will be responsible for performing routine experiments in a research laboratory and contributing to general maintenance of the laboratory, equipment and supplies.
The individual will carry out experiments under the initial supervision until proven proficient in performing experiments independently. An ideal candidate will be motivated to learn new skills, including organoid culture and cell culture experiments. Will perform lab procedures including PCR, DNA and RNA extractions, sample processing and storage, data entry, presentations of results to other researchers, and some data analysis procedures.
Duties and Responsibilities:
Maintain enteroid cell lines in tissue culture environment following established protocols
Bacterial culture (BSL2 microorganisms)
Nucleic acid isolation
Quantitative RT-PCR (qPCR)
Phase-contrast and fluorescence microscopy
Compile, analyze, and interpret research data using computer software applications.
Prepare summary of experimental protocols and relevant data to present/discuss with the faculty investigator
Apply previous knowledge to troubleshoot experiment
Keep a thorough and organized laboratory notebook to document experiments.
Maintain an orderly laboratory workspace.
Ensures laboratory efficiency by following set protocols for lab cleanliness, organization, and safety regulations.
Bachelor's degree in biology, chemistry, or related field. Some related post-undergraduate work experience preferred.
Preferred Qualifications: Previous experience in lab-based science.
Physical Requirements: Sitting, standing and walking for extended period. Reaching by extending hand(s) or arm(s) in any direction. Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s). Ability to move standard equipment through a hospital or clinical/laboratory environment.
Working Conditions:Possible exposure to communicable diseases.
Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $14.14 - $19.44/Hour; Commensurate with experience Employee Group: Full Time Schedule: Monday - Friday, 8:30am - 5:00pm Exempt Status: Non-Exempt Location: School of Medicine, East Baltimore Campus Department Name: Medicine, Gastroenterology Personnel Area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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