The RA I position is part of the research group of Dr. Guoqiang Gu in the Department of Cell and Developmental Biology at Vanderbilt University, School of Medicine. This RA I will be responsible for conducting basic research in the field of diabetes. Reporting directly to, and under the supervision of, Dr. Gu and lab manager Ruiying Hu, the RA I is expected to perform basic procedures in molecular biological, cell biological, and physiological studies primarily in mouse models. The specific roles of this position include: basic DNA cloning, cell culture, mouse handling/genotyping, tissue fixation, sectioning, and immuno-assays. The RA I is expected to be able to prepare some basic solutions/reagents for regular lab operation. He/she is expected to accurately observe, record, and then report the findings to Dr. Gu and/or manager Hu. Motivations to move up to higher level of moreindependent projects is also expected.
The Department of Cell and Developmental Biology is a vibrant, interdisciplinary environment for cuttingedge research over a scale that spans seven powers of ten, from single molecules to whole organisms. The difference between a test tube and a cell is spatio-temporal organization, and we study molecular, cellular and tissue organization in many of the laboratories within our department, seeking insights into fundamental biological questions and human disease. We are an interactive and highly collaborative department, with a strong graduate student association, outstanding core facilities, a top- ranked developmental biology program, and exceptionally strong faculty. Recent faculty recruits are studying cell migration using super-resolution microscopy, single molecule analysis of microtubule dynamics, systems biology of intestinal epithelia, stem cell biology, and the regulation of plasma membrane composition and ageing.
Duties and Responsibilities
Under minimal-to-moderate supervision of Dr. Gu and/or Hu, this RA is expected to:
Prepare buffer/working solutions with proper concentrations and pH for routine lab operation.
Maintain routine cell cultures and help with DNA plasmid construction and preparation.
Manage multiple mouse lines to obtain mice with desired genotypes. These include intercross monitoring, genotyping, and diabetes assays. It is expected that this RA will be able to accurately document/report findings to his/her supervisors.
Perform mouse euthanasia followed by tissue collection and gene expression assays. These include RNA-based assays and immunoassays, involving RNA preparation/RT-PCR and tissue fixation/section followed by immunoassays, respectively. The RA is expected to learn PCR and imaging techniques (mostly microscopic) to accurately document results.
Additionally, the person holding this position is expected to handle multiple tasks and be able to work with different personnel in the laboratory.
Internal Number: 2001039
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.