Research - Laboratory/Non-Laboratory, Staff/Administrative
Duties are as followed:
60%: Perform complex weekly experiments, analyze results and maintain appropriate record. Laboratory techniques may include all of the following:
Transduction of primary immune cells for development of a cell therapy product, flow cytometry, RT PCR, immunohistochemistry, in situ tetramer staining, in situ hybridization, RNAScope, confocal and light microscopy, image analysis, western blotting, animal (mouse) handling
Prepare for and successfully execute experiments with minimal guidance from a supervisor
Follow existing laboratory protocols and independently develop new protocols when needed
Function as a member of a team in planning and executing large studies
Maintain a laboratory notebook
Maintain a working knowledge of the literature relating to the research projects
Analyze data from experiments and present results in appropriate format at lab meetings, in conferences with collaborators, and for grants and publications
Train colleagues with less experience in laboratory protocols
25%: Maintenance and production of stocks and reagents for the laboratory. Laboratory techniques may include all of the following:
Culturing and maintaining cell stocks, preparation of plasmid DNA from bacterial cultures, genomic DNA and mRNA isolation, preparation of retrovirus or lentivirus for cell therapy, isolation of primary immune cells
Production of high quality reagents for the use of other researchers in the laboratory
Careful documentation of quality control tests of the reagents
Maintenance of a database of the laboratory produced reagents
15%: General laboratory maintenance:
Maintain laboratory equipment
Order and maintain laboratory supplies including evaluating products and vendors and obtaining quotes
Maintain laboratory databases
Act as the laboratory biosafety officer
All required qualifications must be documented on application materials.
A minimum of BA/BS and 1 year of relevant experience to equal 5 years or a combination of education and work experience to equal 5 years
The ability to work with tissues or cells from animals infected with viruses or prions
Demonstrated productivity with minimal supervision supported by references
Prefer a bachelor’s degree in the biological sciences with a working knowledge of immunology.
Prefer proficiency in DNA extraction, transfections and transductions, confocal microscopy, experience with flow cytometry, and experience with culture of lymphocytes.
Proficiency with Fluoview, Photoshop, Prism and FlowJo preferred.
Internal Number: 338142
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.