Research - Laboratory/Non-Laboratory, Staff/Administrative
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The UW Department of Surgery/UW Medicine Regional Burn Center has a long history of research into treatments that alleviate many of the medical problems associated with a burn injury. This position will participate directly in the lab with models and experiments as well as work collaboratively with the team on qualitative data analyses across various projects.
The University of Washington (UW) Regional Burn Center in Seattle, Washington is the site for the Northwest Regional Burn Model System (NWRBMS). This burn center is located at Harborview Medical Center in Seattle, serving the people of the Pacific Northwest to include Washington, Alaska, Montana, and Idaho (WAMI region). Harborview is a designated Level I General, Pediatric, and Rehabilitation Trauma Center institution. The NWRBMS (formerly the UW-Burn Injury Model System) has been a funded Burn Model System institution since 1993, with Dr. Nicole Gibran as the current project director of the NWRBMS. Collaborating team members include Dr. Tam N. Pham, Dr. Barclay Stewart, Gretchen Carrougher MN, RN, Claudia Baker BS, and Stephen Sibbett BS.
This position supports several research projects. The NIH Fibroproliferative RTI study relates to investigations into cellular mechanisms of wound healing and modulation of the wound healing process. The BMS studies include Virtual-environment Home Rehabilitation, Early Post-Injury and In-hospital Predictors of Functional Outcomes after Burn Injury and Genetic Predictors of Outcomes after Burn Injury.
DUTIES & RESPONSIBILITIES Research Study Management – 90%
Isolate and purify DNA and RNA from human saliva samples and porcine tissue samples;
Perform molecular biology assays and analyses, including ELISA, Westerns, FACS, and RT2qPCR;
Perform image analysis for in vitro assays to be correlated with in vitro and in vivo Transcriptome data
Maintain large detailed database of sample inventories and protocols;
Perform statistical analysis of data and prepare graphs and tables for presentations;
Understand and comply with all applicable EH&S, IRB, IACUC and other governing regulations.
Work with Research Nurse Supervisor to recruit participants and collect patient reported outcomes data for the BMS program.
Validate data entry for the burn model system national database.
Technical Leadership; Networking/Team Work - 10%
Independently perform experiments with guidance from PI and more senior research staff, but with limited supervision;
Collaborate with co-workers and contribute fully as a member of the team;
Monitor work of students and lab helpers.
JOB QUALIFICATIONS Education: Bachelor’s Degree in biology, molecular biology, chemistry or other field of science that is applicable Type and Years of Experience: At least one year of experience with laboratory research methodologies.
Specific knowledge, skills and abilities required to perform the job satisfactorily include:
Must have a working knowledge of basic computer programs (Word, Excel, PowerPoint)
Must be willing to work flexible hours
Good verbal and written communication skills
Attention to detail and good work ethic
Other Required Qualifications
Molecular biology techniques (ELISAs, Westerns, DNA and RNA isolation, RT-PCR)
Cell culturing techniques
Basic computer programs
Ability to work in a team environment with effective communication skills
Working experience with in vitro collagen contraction assays
Experience with Image analysis techniques and data visualization tools
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.