The Research Assistant will take part in ongoing work funded by the Joint BioEnergy Institute. This project seeks to address the important and challenging task of developing new microbial hosts for biofuel production. The Research Assistant will directly contribute to bench-level experimental research involved in microbial strain engineering and optimization to unlock the potential of renewable energy and other biologically derived materials from single cell organisms, such as Pseudomonas putida.
What You Will Do:
DNA design, manipulation, and cloning.
Engineering of bacterial strains such as Pseudomonas putida, Corynebacterium glutamicum, and Escherichia coli with classical and next generation editing methods, such as homologous recombination and CRISPR.
Functional expression and evaluation of candidate pathway genes in microbial hosts, especially in the context of Sartorius 2L bioreactor scale-up systems (and similar).
Growing, manipulating and archiving bacterial cultures.
Metabolite and proteomic sample prep for LC- and GC-MS.
Additional Responsibilities as needed:
Data recording and report preparation for supervising scientists.
Participating in community laboratory jobs at the RA level. (preparation of antibiotics, buffer/media preparation)
Miscellaneous tasks required to ensure a functional research team. (preparing competent cells, ordering supplies, etc.)
What is Required:
B.S. degree in microbial or molecular biology preferred with demonstrated success in applying molecular biology research techniques.
Working experience in a fast-paced biotechnology startup environment. (preferably at least six months)
Experience in the following techniques: PCR; DNA sequence analysis; DNA isolation, gene cloning and expression; genetic manipulation and cultivation of E. coli or Pseudomonas putida.
Familiarity with recent cloning methods. (Gibson, HiFi assembly, Cas9/CRISPR etc.)
Strong analytical and communication skills.
Ability to be cooperative, team-oriented, mission-oriented, and maintain an up-to-date lab notebook. Must adhere to all campus and EH&S safety policies and model safe lab best practices for others.
Additional Desired Qualifications:
Experience with bacterial strain growth and genetics in Pseudomonas putida
Familiarity with Sartorius Biostat B bioreactors or Sartorius Ambr250 automated bioreactors
Familiarity with ssDNA oligo recombineering a plus.
The posting shall remain open until the position is filled.
This is a full time, 1-year term appointment with the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs.
M-F, Non-exempt (hourly paid) from overtime pay.
This position is represented by a union for collective bargaining purposes.
Salary will be determined based on range by collective bargaining agreement.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at: Joint BioEnergy Institute (JBEI) -- Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA.
Learn about us!
Biological Systems and Engineering Division
Vision: To lead efforts that combine the power of biology with the tools of engineering to develop sustainable energy and biomanufacturing solutions and improve human health.
Mission:Advance a mechanistic and predictive understanding of complex biological systems over multiple scales in terms of their responses to manipulation, stress, disease and environmental challenges and translate this knowledge using engineering principles to develop resilient systems, tools, and processes for the efficient production of fuels, chemicals, materials, tissues, and therapeutics.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Working at Berkeley Lab has many rewards including a competitive compensation program, excellent health and welfare programs, a retirement program that is second to none, and outstanding development opportunities. To view information about the many rewards that are offered at Berkeley Lab- Click Here.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law.
Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers.
Internal Number: 91273
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.