Research - Laboratory/Non-Laboratory, Staff/Administrative
Summary of Position: Dedicated researcher to join a large and dynamic lab. Design and create recombinant DNA constructs for expression in HEK cells. Grow and maintain mammalian cell lines. Carry out high-throughput assays for heart-failure drugs. Perform basic lab maintenance. Present research at lab meetings.
Essential and Other Functions: 50% Molecular Biology Generate recombinant DNA constructs for creating stable clones using primer design, PCR, ligation, site-directed mutagenesis, restriction digests, and similar techniques. Characterize expression via western blot. Maintain recombinant DNA database and stocks. Design new cloning strategies as needed.
30% Cell Culture Generate, grow and maintain stable cell lines expressing fluorescently labelled proteins in HEK cells. Perform regular quality control tests on cultures and inventory of cell banks.
10% Carry out high-throughput assays for heart-failure drugs. Preparation of reconstituted fluorescently-labeled membrane proteins, performance of microplate-based assays for enzymatic activity and protein-protein interactions, trouble-shooting and improving functional assays. Employee will be trained in state-of-the-art technology in both absorption and fluorescence spectroscopy using high-density microplate arrays, plus computational and statistical analysis.
10% Lab maintenance and meetings Perform maintenance of laboratory equipment and ordering of supplies and parts. Place orders for needed reagents and materials for experiments. Present research at lab and weekly meetings.
All required qualifications must be documented on application materials.
Required Qualifications: BA/BS in Biology, Biochemistry, Genetics, or related field, or a combination of relevant education and experience to equal four years. Previous mammalian tissue culture experience. Laboratory experience with common molecular biology techniques including site-directed mutagenesis, cloning, primer design, enzyme assays and reagent preparation. Ability to carry out research protocols from written or oral instructions by supervisor.
Preferred Qualifications: Extensive experience with nucleotide sequence analysis and challenging cloning projects highly preferred. Experience with HEK cells preferred and previous flow cytometry experience a plus.
Internal Number: 337870
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.