Job Summary: Conducts tests and procedures; sets up and operates equipment; records observations and measurements and collects and compiles data in a research lab; requires a practical understanding of a limited number of standard scientific techniques.
Basic responsibilities will include the preparation of various gene constructs using current molecular cloning strategies, DNA sequencing submission and results analysis, and the transformation of bacterial strains and their maintenance. Accordingly, applicants should have experience and a strong understanding of the principles and techniques of molecular biology. These include specifically, familiarity with the elements of plasmid design and propagation in bacteria, and gene construction methods such as directional, Golden Gate, and Gibson assembly. Additional responsibilities will involve preparing bacterial cultures for protein expression, inoculation, induction, cell harvesting of expression cultures, protein isolation and purification. Qualified candidates will have experience with at least some of these procedures. Ideal candidates would also have experience preparing protein reagents for use in cell-based experiments and / or experience performing cell-based assays.
This is a one year termed position subject to renewal dependent on performance and available funding
Follows pre-defined protocols, policies, procedure, regulations, Good Clinical and Good Laboratory Practices and conducts standard and repetitive experiments and completes basic trouble-shooting techniques.
Sets-up, operates and maintains standard lab equipment.
Using an entry level of skill, completes the following activities (please highlight):
DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
Cloning, mapping and sequencing of DNA
Cell culture, cell isolation
ELISA, Elispot assay
Antibody production and purification
Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
Records test results and may maintain databases.
Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
Maintains inventory of reagents, mice and lab supplies
Performs other duties as assigned.
Minimum Qualifications: HS diploma or equivalent and some basic science courses is required.
Minimum Competencies: Applicants should have some proficiency with DNA editing, protein expression and purification, and basic word processing and spreadsheet software.
Preferred Qualifications: Applicants should have some proficiency with DNA editing, protein expression and purification, and basic word processing and spreadsheet software.
Preferred Competencies: Cell-based assays and techniques of cell culture and maintenance. Knowledge and proficiency with biophysical techniques such as ELISA, FACS, etc
Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.
Internal Number: 39442
About Northwestern University
Northwestern University is a major private research university with 12 academic divisions located on three campuses in Evanston, Chicago, and Education City in Doha, Qatar. We have approximately 2,500 full-time faculty members, 17,000 graduate and undergraduate students, and over 5,700 full and part-time staff. Northwestern University combines innovative teaching and pioneering research in a highly collaborative environment. It provides students and faculty exceptional opportunities for intellectual, personal and professional growth.