Research - Laboratory/Non-Laboratory, Staff/Administrative
Assistant Scientist - Georgetown University Medical Center
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We are seeking an Assistant Scientist in the Slingerland laboratory, for research in cell cycle regulation and PI3K signaling, to investigate novel pro-oncogenic roles of p27 in transcriptional regulation and epigenetics. Other projects pursue how estrogen receptor and NFKB pathways interact to link estrogens, obesity, inflammation, and breast cancer stem cells. Projects require discretion and judgment in research activities within project scope and methodologies. This position requires in-depth knowledge of molecular biology, protocol/ method development, organizing and implementing complex research plans, analysis of data, and writing manuscripts for publication of findings.
The Assistant Scientist will assist the Principle Investigator to conduct research and may assist the lab manager in organizing purchase of reagents and supplies. He/she will assist with training junior lab members in routine protocols and assisting in all organizational aspects of the laboratory per Georgetown University policies and procedures.
Perform complex laboratory assignments, including the planning and conducting of projects related to cell culture and molecular biology. Schedule and perform experiments in research laboratory.
Solve problems in scientific and technical execution, interpret experimental data and maintain accurate records. May need to develop, adapt and implement new lab procedures.
Provide technical advice and instruction to lab members as needed.
Design and perform complex and routine molecular biology procedures not limited to but including flow cytometry analysis, PCR, RT-PCR, QT-PCR, Southern, RNA Seq, Western blotting, DNA/RNA extraction, recombinant protein isolation and purification, bacculoviral vector protein production and gene cloning, transfection, in vitro translation and affinity chromatography, microscopy and immunohistochemical staining of tissue sections; ChIP Sequencing and epigenetic analyses.?
Assist in the preparation of data and figures for manuscripts and for scientific presentations using Power Point, Adobe Illustrator and other graphics software as needed.
Search literature for references to technical problems and keep informed by reading relevant journal and publications.
Present data in laboratory meeting and at international meetings and other venues.
Other duties as assigned.
Individual hired will need to be able to function independently to manage daily affairs of the research laboratory design and perform routine and complex laboratory research experiments. Will receive instruction and mentorship from the Principal Investigator, Dr Slingerland.
Ph.D. in a relevant field of cellular and molecular research and at least 3 years of post -PhD research experience.
Candidates must possess:
1) broad knowledge of cancer research and experience in human cell/tissue culture, molecular biological techniques, experience with animal models; prior experience in epigenetics/bioinformatics is desirable but not required.
2) ability to plan, organize and meet deadlines is required.
3) must be highly motivated and detail oriented.
4) excellent communication skills (verbal and written), and ability to collaborate with others.
5) ability to successfully manage multiple tasks simultaneously and independently.
6) demonstrate problem solving skills.
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