The Postdoctoral Fellow will report to Dr. Johnson and interact with a collaborative group of researchers studying glycogen storage diseases, infectious diseases, and cancer. For more information on the kind of research conducted by the group, please visit the lab website at https://medicine.iu.edu/faculty/22597/johnson-steven
Main Responsibilities: With a high degree of independence, the successful candidate will conduct a wide range of complex experiments in molecular & cell biology, biochemistry, chemical biology, and organic/medicinal chemistry. Individuals with multidisciplinary experience and a desire to tackle diverse challenges in drug discovery are encouraged to apply. A rough break-down of responsibilities is as follows:
Research (~75%): Primary responsibilities will be to synthesize organic molecules and evaluate their bioactivities in a panel of biochemical and cell-based assays to develop structure-activity relationships and elucidate inhibitor mechanisms of action. Requires skills in chemical synthesis, purification, and analysis by HPLC, NMR, and LC-MS; assay development and compound screening; bacterial and mammalian cell culture; and protein expression and purification.
Lab management (~10%): Perform general duties to ensure the lab operates safely and efficiently, including assisting the PI with monitoring the completion of assigned tasks; ordering supplies / equipment and obtaining product quotes; maintaining equipment, reagents, databases, and coordinating waste disposal; serving as a resource to lab personnel.
Preparation of research reports and presentations (~5%): Assist in preparing and submitting project reports, manuscripts, and grants.
Continuing education (~10%): Maintain knowledge of scientific trends through readings and seminars, and review literature to identify suitable procedures and methods.
At the IU School of Medicine, we offer focused, systematic and sustained programs that are aligned with three foundational pillars of diversity and inclusion: representational diversity, inclusive working and learning environment, and cultural competence. Therefore, we are looking for applicants who can help ensure all our mission areas are inclusive and equitable.
Education and Experience Requirements: A PhD is required, with preferred areas of study in organic/medicinal chemistry, biochemistry, chemical biology, molecular biology, cell biology, or a related field. Practical synthetic organic, medicinal, and analytical chemistry experience in a research setting is required. Enzymatic and cell-based assay development, compound screening, and molecular and cell biology and biochemistry experience are desired. Cloning, bacterial cell culture, mammalian tissue culture, and protein expression & purification skills are advantageous. Willingness to learn and master a variety of laboratory techniques and tackle new challenges in other disciplines as necessary is expected. Excellent communication skills (speaking and writing English) are required.
Required knowledge, skills, and abilities:
Synthetic organic, medicinal, and analytical chemistry skills are required. Retrosynthetic analysis to develop procedures for synthesizing target compounds is necessary. Ability to synthesize compounds from mg to g scale using inert atmosphere techniques, and purification via normal and reverse-phase chromatography is expected. The ability to analyze and characterize compounds by RP-HPLC, LC-MS, and proton/carbon NMR is required.
Preference will be given to those with knowledge, skills, and abilities evaluating compounds in enzymatic and cell-based assays (including radioactivity-based detection); protein expression and purification; and bacterial and mammalian cell culture.
Excellent organizational skills and independent scientific problem solving abilities are required.
Experience with software including ChemDraw, ChemFinder, MestReNova, CLC Sequence Viewer, Discovery Studio, SoftMax Pro, Waters Empower 3, GraphPad Prism, SciFinder Scholar, Microsoft Windows and Office (Word, Excel, PowerPoint), and EndNote is beneficial.
Must adhere to chemical and biological safety practices within a laboratory research setting.
Excellent communication skills and ability to work well with others.
Equipment utilized: General research lab equipment including balances, fumehoods, fridges, freezers, pH meters, centrifuges, incubators, shakers, pipets, etc. More specialized equipment for chemistry duties includes rotovaps, Biotage Isolera flash chromatography systems, Biotage microwave synthesizers, HPLCs (analytical and preparatory), lyophilizers, NMRs, and LC-Mass Specs. Additional equipment includes autoclaves, biosafety hoods, microplate dispensers and readers, thermocyclers, and FPLCs.
Department Contact for Questions:
Name: Steven M. Johnson Phone: (317) 274-2458 Email: firstname.lastname@example.org
OAA #: IUSM009142016
Internal Number: 9905
About Indiana University
Founded in 1820, Indiana University is the state’s public university system, comprised of two core campuses—IU-Bloomington and IUPUI—and six regional campuses located throughout the state. Combined, the total student population is more than 114,000 undergraduate, graduate and professional school students. IU is one of the largest employers in the state, with over 20,000 faculty and staff. Indiana University is an equal employment and affirmative action employer and a provider of ADA services. Diversity and inclusion remains a core commitment to IU’s mission to cultivate an educational environment that supports equal access, participation and representation on all of its campuses and that provides educational and career opportunities for all.