The research of Lena Heung, MD, PhD, focuses on the roles of innate immune cells in the host response to respiratory fungal pathogens, in particular Cryptococcus neoformans. C. neoformans is an environmental fungus that can cause highly fatal lung and central nervous system infections in immunocompromised patients, including those with HIV/AIDS, cancer, solid organ transplantation and autoimmune conditions. The goal of Dr. Heung's research is to define the innate immune mechanisms that determine host outcomes after infection in order to facilitate the development of immunomodulatory therapies for susceptible patients. The Heung Lab has established detrimental roles for inflammatory monocytes and signaling through the DAP12 adapter molecule during cryptococcosis. Currently, the lab is investigating the cellular and molecular mechanisms by which inflammatory monocytes regulate the host response to C. neoformans and further dissecting the DAP12 signaling cascade and its role in integrating fungal sensing and downstream effector functions.
Working under direct supervision, as a Research Associate II, you will perform routine and increasingly complex laboratory tasks and procedures and develops technical expertise in one or more areas relevant to research projects. You will participate in research activities involving all aspects of the research process, to include, but not limited to; investigation, technical, testing/validation of results, and report findings. Will perform a variety of routine experimental protocols and procedures to support the objectives of laboratory research projects and will observe and comply with safety standards and procedures, assist in preparation of data for publication and/or presentation at scholarly meetings, and assist in general laboratory maintenance. This role does not have supervisory responsibilities.
Essential job duties and responsibilities:
Performs a variety of increasingly complex experimental protocols and procedures and assist in the design of new complex or unusual protocols and techniques.
Keeps accurate and detailed records of experiments and results and assists in identifying and troubleshooting of unexpected results.
Orders laboratory supplies and supports laboratory operations.
Maintains lab equipment and related records and may coordinate use of lab equipment.
Assists in the operation of specialized equipment, as required by the area of research.
Assists in preparation of data for publication and/or presentation at scholarly meetings.
Observes and complies with safety standards and procedures.
Orients research laboratory assistants or lower level research associates and undergraduate student researchers on day-to-day lab operations and routine procedures.
Department and/or unit specific responsibilities:
Organize and maintain mouse lines specific to the lab of Dr. Lena Heung.
Perform animal work including infections, injections, and tissue harvest as well as breeding, weaning, and genotyping of transgenic and knockout animals.
Other duties may include: Tissue culture, preparation of microbial cultures, ELISA, Western blotting, quantitative RT-PCR, flow cytometry, immunohistochemical and quantitative analysis of tissue samples, organization and maintenance of samples and other laboratory stock material.
Providing healthcare for more than 100 years, Cedars-Sinai has evolved into one of the most dynamic and highly renowned medical centers in the world. Along with caring for patients, Cedars-Sinai is a hub for biomedical research and a training center for future physicians and other healthcare professionals. This attracts exceptional talent to Cedars-Sinai, including world-renowned physician-scientists who seek a place where they can both conduct research and see patients--the ideal formula for discovery and its translation into cures. Our patients benefit from access to doctors at the top of their fields, and our researchers have an ideal community in which to study the impact of healthcare challenges, and reflect that knowledge in their research. The greater Los Angeles area in which Cedars-Sinai resides possesses unparalleled cultural and ethnic diversity which offers outstanding opportunities for translational and clinical research and a dynamic environment for medical education.Although community based, Cedars-Sinai is a major t...eaching hospital affiliated with the David Geffen School of Medicine at the University of California, Los Angeles (UCLA). Cedars-Sinai has highly competitive graduate medical education programs in more than 50 specialty and subspecialty areas, a graduate program in biomedical sciences and translational medicine, a clinical scholars program directed towards junior physicians with aspirations to become clinical scientists, and post graduate training opportunities.There are more than 250 full-time faculty members at Cedars-Sinai. The voluntary medical staff, comprised of more than 2,200 specialty board-certified or board-qualified physicians, represent all of the specialties and subspecialties and collaborate with full-time medical staff in the teaching responsibilities of the graduate medical education programs.