Research - Laboratory/Non-Laboratory, Staff/Administrative
Summary of Position: This position in the Bushley Lab will primarily focus on research related to investigating sources of genetic resistance in the cover-crop pennycress. Responsibilities will include growing plants in tissue culture and inoculating with the soybean cyst nematode, maintaining soybean cyst nematode stocks on soybean plants, screening candidate genes using qPCR, and analyzing RNA-Seq data. The technician will also have responsibilities in general lab maintenance (inventory and safety compliance) and other experiments including fungal-nematode interaction assays and DNA and RNA extractions.
Essential and Other Functions: 50% Research investigating succeptibiity of different Pennycress and Arabidopsis lines to the soybean cyst nematode and the effects of different fungi in deterring colonization. Conduct in-vitro bioassays, qRT-PCR, microscopy and tissue staining and quantifying nematodes in root systems.
20% Investigate effects of fungi in interaction with the SCN Conduct in-vitro fungal-nematode and fungal-fungal interactions studies, perform RNA extractions and extractions for metabolomics experiment.
15% Analyze data, keep good records, and assist in writing manuscripts Maintain records of experimental data, conduct statistical and bioinformatics analyses of results, and assist PI in writing of project reports and manuscripts.
10 % General lab maintenance and safety compliance Keep inventory and order commonly used laboratory supplies, perform chemical and biological safety checks and hazardous waste disposal.
5% Train other researchers Provide training in techniques to other researchers, particularly undergraduate or graduate students working on the project
Essential Qualifications; A BS/BA in biological sciences, microbiology, plant pathology, plant breeding, genetics, or related fields and 1 year of related experience or equivalent combination of education and work experience to equal five years. Previous experience working in a biological research lab with fungi and familiarity and skill with basic molecular biological techniques such as DNA and RNA extractions.
Preferred Qualifications: A master’s degree or post-baccalaureate research experience preferred. Skills in microscopy and experience working with Arabidopsis and plant tissue culture desirable.
Internal Number: 337496
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