Research - Laboratory/Non-Laboratory, Staff/Administrative
40% General Mouse Maintenance Daily/Weekly: Under the supervision of Research Associate or Postdoctoral fellow, the duties will include handling mice, setting breeding, weaning pups, tail cutting and tagging mice, DNA extraction from mouse tails or ear punch and PCR analysis of transgene. The person is also required to update the mice database updated for the mice lines being maintained.
Intermittent: Performing euthanasia and collecting tissues for biochemical and/or histological analysis. Administration of experimental compounds via injection (ip or sc) or oral delivery. Working with the mouse behavioral core to characterize Motor and cognitive behavior of the mouse lines.
50% Perform Experiments Biochemical experiments: Responsible for basic biochemical analysis of protein expression using immunoblot analysis. Immunoblot analysis includes tissue homogenization, determination of protein concentration, SDS-PAGE, Western Blot and use of Gel Imaging System. Aid in obtaining recombinant proteins (growth of E. Coli, Cell lysis, Chromatography).
Tissue Culture: Duties include routine tissue culture and passage of cell lines, freezing of the cell lines, and establishing and maintaining primary cultures of brain cells.
10% General Laboratory Maintenance and professional development Duties include, but not limited to, organization of laboratory supplies and environment, replenishing common buffers, and learning new techniques as needed.
Required Qualifications (must be mentioned on application and/or resume): Bachelor's Degree in Neuroscience or related field (e.g. Biology, Biochemistry, cell biology etc), or a combination of related education and work experience to equal four years.
Must be willing to work with mouse models of Alzheimer's disease and Parkinson's Disease.
Preferred Qualifications: Laboratory experience in cell biological, biochemical, and/or neurobehavioral techniques. These might include, but not limited to, working with transgenic mouse models and genotyping, cell culture, protein chemistry and protein gel electrophoresis, and/or immunohistochemistry.
Internal Number: 337373
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