The Research Assistant I is part of the Chen lab in the Department of Molecular Physiology & Biophysics (MPB) at Vanderbilt University and is a key individual contributor responsible for responsible for assisting the Principal Investigator in various aspects in research and management. Reporting directly to the Principal Investigator, the Research Assistant I generates and maintains transgenic and mutant zebrafish lines and cell culture for lentivirus production and lentivirus-based genetic analysis. The managerial responsibilities include ordering and organizing lab supplies.
The Chen lab consists of a culturally diverse team of postdoctoral fellows, graduate students, and Research Assistance. The lab is very collaborative both within and outside of the laboratory. It is part of MPB and the Vanderbilt Diabetes Research and Training center, both are international leaders in diabetic research.
Duties and Responsibilties
Generation and maintenance of zebrafish lines
Generation and characterization of transgenic and mutant lines including transgene production and sgRNA synthesis, microinjection of zebrafish embryos, and screen for transgenic and mutation carriers using fluorescent microscopes, PCR genotyping, and sequencing;
Organizing and tracking various transgene- and mutation-carrying lines.
Freezing and depositing transgenic and mutant lines as needed.
Genetic analysis in mammalian pancreatic beta-cell lines.
Genetic screen in mammalian pancreatic beta-cell lines
Ordering and maintenance of research supplies.
Organization of lab.
Internal Number: 2000702
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.