The Research Assistant is a member of the Cell and Developmental Biology Department within Basic Sciences at Vanderbilt University and is a key individual contributor responsible for using Fluorescence Activated Cell Sorting (FACS) to isolate specific C. elegans neurons from dissociated preparations of larval animals. The individual will also participate in a research project that uses molecular genetics, behavioral analysis and live cell imaging to investigate synapse formation in the nervous system of C. elegans. Reporting to the Principal Investigator, the Research Assistant interacts with students, staff, and faculty within the laboratory.
This position will provide an opportunity to learn methods for Fluorescence Activated Cell Sorting (FACS), molecular genetics, behavioral analysis and live cell imaging. Additional opportunities include the operation of fluorescence microscopes to monitor GFP expression in nematode cultures, maintenance of transgenic nematode strains and databases. The RA1 will assist with daily operation of the laboratory (e.g. ordering supplies, maintaining equipment, assist undergraduate students in media preparation, plate pouring, etc.).
The Department of Cell and Developmental Biology is a vibrant, interdisciplinary environment for cutting-edge research over a scale that spans seven powers of ten, from single molecules to whole organisms. The difference between a test tube and a cell is spatio-temporal organization, and we study molecular, cellular and tissue organization in many of the laboratories within our department, seeking insights into fundamental biological questions and human disease. This year we achieved the #3 ranking in the nation for funding, as compared with similar departments in research institutions and universities. We are an interactive and highly collaborative department, with a strong graduate student association, outstanding core facilities, a top-ranked developmental biology program, and exceptionally strong faculty. Recent faculty recruits are studying cell migration using super-resolution microscopy, single molecule analysis of microtubule dynamics, systems biology of intestinal epithelia, stem cell biology, and the regulation of plasma membrane composition and aging.
Duties and Responsibilities
Uses FACS to isolate specific C. elegans neurons for RNA-Seq analysis; maintains nematode cultures.
Use molecular genetics, behavioral analysis and live cell imaging to investigate the formation of
synapses in the nervous system of C. elegans.
Works with Laboratory Manager to maintain freezers, prepare competent cells, ship nematode
strains and reagents to other laboratories, order supplies and maintain equipment.
Prepares sterile media, pour nutrient agar plates, inoculate bacterial cultures.
Internal Number: 2000640
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.