Research - Laboratory/Non-Laboratory, Staff/Administrative
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The Department of Genome Sciences (GS) is part of the University of Washington School of Medicine (UW SoM) and is comprised of 27 labs addressing leading edge questions in biology and medicine by developing and applying genetic, genomic and computational approaches taking advantage of genomic information now available for a host of species. GS is making major contributions in the application of genome science to understand the fundamentals of biology and to improve human health and wellbeing. Genome Sciences is a collaborative department supported by very different academic disciplines. We work with a multitude of Program Investigators (PIs) in departments both on the UW campus as well as worldwide. The department has gone through significant expansion since its beginning in 2001 with annual grant awards of $56M in FY19.
The Department of Genome Sciences currently has an outstanding opportunity for a Research Scientist/Engineer 2.
Position Purpose: The Research Scientist/Engineer 2 will provide technical support for several different projects simultaneously for the Principal Investigator (PI), postdoctoral fellows, and graduate students. Projects include investigating the role of genomic structural variation in human disease and primate evolution, genetic architecture of autism, and understanding natural genome variation between humans and other primates. Techniques used will include next-generation and long-read DNA sequencing, genomic clone characterization, and gene/protein characterization.
This position will work in a prominent laboratory whose work impacts other researchers worldwide. High functionality will be reflected in even greater funding opportunities and enhancement of the reputation of the University as a biomedical research powerhouse.
Assist in the development and implementation of investigative procedures to accomplish research goals. Independently perform scientific investigative procedures. Analyze and interpret experimental results and assist in determining relevance to project. Prepare reports, charts, and graphs directed at communicating results at scientific meetings or in preparing manuscripts for publication. Actively contribute to the research results of the laboratory. Work collaboratively with other technicians; assist graduate students and postdoctoral fellows on multiple ongoing research projects.
Support single-molecule, real-time (SMRT) and Oxford Nanopore (ONT) sequencing projects, including library preparation, sequencer operation, data quality monitoring, and customer interaction duties
Select, prepare, sequence, assemble, and keep records of clones for PacBio sequencing
Sample tracking and database maintenance
DNA and RNA isolation from various sources
Prepare samples and create libraries for sequencing on the Illumina and Sanger platforms and analyze the data
Operate and troubleshoot Illumina next-generation sequencing platforms, including the NextSeq 500 and MiSeq
Perform routine analysis of data: assist with troubleshooting and daily quality control of data and assist researchers with data analysis, organization and interpretation, and experimental design
PCR amplification from genomic DNA samples
Handle bacterial and human cell cultures, including clone picking, streaking agar plates, making stocks, and miniprep DNA isolation
Perform sequence analysis using an interface and on the command line
Contribute data for reports and publications
Provide status reports (twice monthly) to the PI
Perform related duties as required
Bachelor’s degree in Biology, Biochemistry, Microbiology or related Life Sciences field and two or more years related experience in molecular biology or human genetics
Some familiarity with next-generation sequencing technologies
Experience and knowledge of the fundamental concepts, practices, and procedures required for working in a genomics laboratory
Able to work independently and as part of a multidisciplinary team
Able to learn new techniques
Self-motivated with the ability to take initiative and work independently
Excellent communication skills
Exact attention to procedural detail
Meticulous record-keeping skills and organizational abilities
Must have basic computational skills to store, analyze, and present data using Microsoft Word, Excel, and PowerPoint, as well as Google Docs and Sheets
Master’s degree in Microbiology, Genetics or related Life Sciences field
Experience with next-generation sequencing technologies
PCR & qPCR for genotyping
DNA & RNA isolation
Familiarity with long-read sequencing
Cell line maintenance
Background in sequence analysis and programs such as Sequencher or IGV
Experience in team-focused work environment
Experience with the genome browser and BLAT/BLAST and familiarity with Human Genomics terms and concepts
Basic knowledge of working from the command line
Experience with UNIX/*NIX command line and BASH shell scripting is strongly desired
BAC-insert sequencing and assembly
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.