In this exciting role, you will study the structure and function of macromolecular complexes and enzymes using biochemical, X-ray crystallographic, and electron cryo-microscopy (cryo-EM) methods. You will be part of the Structural Biology group of the Technology Division at the Joint BioEnergy Institute (JBEI) in Emeryville, California. Examples of the biological systems to be studied are:
Glycosyl transferases responsible for the synthesis of hemicelluloses in plants.
Novel glycosyl hydrolases that are able to break down cellulosic material for biofuels production.
Enzymes involved in lignin biosynthesis, degradation, and valorization.
Secondary metabolite and natural product enzymes.
You will have experience characterizing enzymes using biochemical techniques, and familiarity with structural biology methods, such as crystallography or cryo-EM. Robotic hardware for performing crystallization trials and imaging results are available. Crystals will be characterized and data collected using the beamline resources of the Berkeley Center for Structural Biology at the Advanced Light Source. For appropriate systems cryo-EM data will be obtained using in-house microscopes, the Bay Area Center for Cryo-EM, and other national user facilities.
What You Will Do:
Ability to work independently with high-level guidance from the project lead.
Biochemical characterization of enzymes using standard assay techniques, including analysis of enzyme kinetics, substrate binding, and protein stability.
Performing screens for crystallization conditions.
Optimization of crystallization conditions.
Biophysical characterization of protein samples using standard techniques, such as dynamic light scattering.
Characterization of crystals using synchrotron beamlines.
Collection and analysis of diffraction data, model building and structure refinement.
Characterization of samples using cryo-EM methods when samples permit.
Extensive email and verbal interaction with other researchers.
Additional Responsibilities as needed:
Use of molecular biology methods to prepare expression constructs.
Protein expression and purification.
Use of small-angle X-ray scattering methods to analyze macromolecular complexes in solution.
What Is Required:
Ph.D. or equivalent experience in a scientific discipline, preferably structural biology, biology, or chemistry.
Demonstrated experience at the postdoctoral level of scientific research using biochemistry techniques.
Ability to work independently with high-level guidance from the project lead.
Ability to troubleshoot problems and identify new approaches to achieve technical goals.
Familiarity with crystallographic methods.
Experience with macromolecular crystallization techniques.
Solid interpersonal skills and the ability to work in a team environment are critical.
Ability to communicate with a broad range of researchers.
Additional Desired Qualifications:
Experience with molecular biology and protein expression methods.
Experience with the use of small-angle scattering methods.
Familiarity with robotic hardware for crystal growth.
Familiarity with cryo-EM methods.
The posting shall remain open until the position is filled.
This is a full time, 3 year, term appointment with the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs.
M-F, exempt (monthly paid) from overtime pay.
Salary is commensurate with experience.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at Joint BioEnergy Institute (JBEI) -- Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA.
Learn about us!
Molecular Biophysics and Integrated Bioimaging
Mission: To generate a mechanistic and predictive understanding of biological processes, by developing and applying molecular- and meso-scale visualization and advanced spectroscopies, enabling the control, manipulation and generation of biological function.
Vision: To generate fundamental knowledge that inspires a mechanistic understanding of biological processes, with the ultimate goal of manipulating, controlling, and creating biological functions in order to solve national challenges in energy, environment, health and biomanufacturing.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Working at Berkeley Lab has many rewards including a competitive compensation program, excellent health and welfare programs, a retirement program that is second to none, and outstanding development opportunities. To view information about the many rewards that are offered at Berkeley Lab- Click Here.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law.
Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers.
Internal Number: 90561
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.