The NF-kappaB family of transcription factors are regulators of many critical processes such as proliferation, apoptosis, immune responses and inflammation. It is, therefore, unsurprising that they play roles in basic development but also in most diseases including cancer. Our laboratory has generated a number of mouse models to investigate these roles in development; breast, lungs, limbs and immune system. We are also interested in the roles played by NF-kappaB signaling in disease. We are currently primarily interested in ovarian, breast and lung cancer. We have generated a number of interesting models that we use for our research. Our transgenic reporter mice express a GFP/luciferase fusion protein that can emit light in response to NF-kappaB activity. With these we can monitor activation in a living animal using a CCD camera and use immunohistochemical techniques to identify specific cell types in which NF-kappaB is active. We have also generated a modular inducible system that enables us to turn up or down NF-kappaB activity in specific cell types eg. mammary or lung epithelium or macrophages. We are using multiple models to study the effects of modulating NF-kappaB activity on normal breast development, early stages of primary breast tumor development and on metastasis to the lungs. We are also collaborating with Professor Todd Giorgio in the Department of Biomedical Engineering to develop nanoparticles that deliver siRNA to macrophages for cancer therapy.
Duties and Responsibilities
Assisting with experiments including maintaining accurate and complete experimental records
Mouse husbandry and genotyping
Placing orders through Vanderbilt eProcurement and CORES/iLabs
Preparation of laboratory reagents
Maintaining a clean, organized laboratory space.
Good communication skills
Able to learn new techniques quickly
Maintain detailed experimental records
Work with minimal supervision after appropriate training
Profile of an Ideal Candidate
Bachelors degree is required
Strong interpersonal and communication skills and the ability to work collaboratively with a wide range of constituencies in a diverse community is strongly preferred
Experience preferred in: mouse husbandry, genotyping and/or tumor induction; cell culture; immunohistochemistry, flow cytometry, Vanderbilt eProcurement and CORES/iLabs is preferred
Vanderbilt experience is preferred
Commitment to Equity, Diversity and Inclusion
Vanderbilt University is committed to achieving the goal of a diverse and inclusive academic community of faculty, staff, and students. We seek individuals who are committed to this goal and our campus values.
Internal Number: 2000447
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.