Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutchs pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nations first cancer prevention research program, as well as the clinical coordinating center of the Womens Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
The Riddell lab in the Program in Immunology in the Clinical Research Division is looking for a highly motivated individual to support the development of CAR-T cell immunotherapy for cancer. The successful candidate will assist with design, execution, and analysis of pre-clinical studies using murine tumor models to study strategies for improving CAR-T cell therapy. They should be capable of handling murine, maintaining detailed laboratory records, analyzing data, managing supplies and reporting experimental results in a fast-paced environment.
This position will work under the direction of a postdoctoral fellow and will be involved in the following techniques/duties:
Perform sterile tissue culture techniques, including primary T cell culture, transduction of murine cells with retroviral/lentiviral vectors, FACS cell sorting
Assist with in vivo murine tumor model experiments, including monitoring weight, body condition score, intravenous (retro-orbital or tail vein) injection, intraperitoneal injection, and subcutaneous injection
Dissect and process various tissues, including spleen, peripheral lymph nodes, bone marrow, lungs, and tumors
Analyze cell phenotype and function from in vitro and in vivo experiments using multi-color flow cytometry, RT-PCR, and immunohistochemistry
Prepare samples for analysis by next-generation sequencing methods, including single-cell RNAseq
Familiarity with molecular biology techniques, including bacterial plasmid preps, PCR, and cloning
Perform in vitro assays examining T-cell cytotoxicity, cytokine production and proliferation
Maintain detailed laboratory records
Bachelor of Science degree in a biological science and 2+ years of hands-on bench research experience
Experience handling murine
Detail-oriented, motivated, well-organized and have good attention to detail
Ability to communicate effectively and keep accurate records of work performed
Ability to work on a team
T cell tissue culture and multi-color flow cytometry are highly desirable
Experience in murine injections (IV, IP) and tissue dissection are preferred
Background in immunology or T cell biology in particular, is preferred
Computer skills: Microsoft Excel, Prism (Graphpad), FlowJo
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At Fred Hutchinson Cancer Research Center, teams of world-renowned scientists and humanitarians work together to prevent, diagnose and treat cancer, HIV/AIDS and other diseases. Researchers are discovering new ways to detect cancers earlier, improve treatments, and learn how to prevent cancers from growing. Although Fred Hutchinson opened its doors in 1975, its history began about 20 years before that. In 1962 Fred Hutchinson envisioned a center devoted to studying cancer, a disease that took his brother’s life. Today Fred Hutchinson is contributing to the next waves of breakthrough treatments and prevention strategies. Fred Hutchinson collaborates with the Seattle Cancer Alliance, the National Cancer Institute, and the University of Washington.