Research - Laboratory/Non-Laboratory, Staff/Administrative
Washington University is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, age, sex, sexual orientation, gender identity or expression, national origin, genetic information, disability, or protected veteran status.
This position is full-time and works approximately 40 hours per week.
Department Name/Job Location:
This position is in the Department of Internal Medicine, Division of Oncology. This position is for the Medical School Campus.
There is an opportunity for research in the laboratory of Dr. Jieya Shao. The central theme of the lab is unravelling novel cancer biology with the overarching goal of developing effective therapies. Two main projects are currently ongoing primarily in the context of breast cancer (www.shaolab.org).
“Moonlighting” functions of the actin-binding protein profilin-1 (Pfn1) in transcriptional and epigenetic regulation: As an essential eukaryotic protein, the mechanisms behind the paradoxical anticancer effects of profilin-1 have remained mysterious for nearly two decades. Our recent studies suggest that the anticancer activities of Pfn1 may stem from its poorly understood nuclear functions which become deregulated in cancer due to an alteration of its nucleocytoplasmic trafficking. We are currently focusing on defining its novel roles in transcriptional control and epigenetic regulation in the context of both estrogen receptor positive and triple negative breast cancer by utilizing multidisciplinary approaches including biochemistry, molecular and cellular biology, proteomics, mouse models, and next generation sequencing
The role of VCP in protein homeostasis, DNA damage response and chemotherapy efficacy of cancer: Valosin-containing protein (VCP) is an evolutionarily conserved and highly abundant AAA+ ATPase involved in diverse cellular processes. Functioning as a “segregase”, VCP maintains protein homeostasis by extracting polyubiquitinated proteins from various cellular locations or complexes for turnover. Numerous studies have now linked VCP to chromatin-associated protein degradation and genome stability maintenance particularly in the context of DNA damage response. Nevertheless, detailed understanding of its mechanism of action, its mode of (de)regulation in cancer, and its relevance to chemotherapy response is lacking, and we are currently investigating all these questions.
Bachelor’s degree or above.
General understanding of biochemistry, molecular and cellular biology.
Prior experience in mammalian cell culture, Western blotting, DNA/RNA isolation, mouse handling and surgery.
Knowledge in genetics/epigenetics and experience in quantitative PCR and chromatin immunoprecipitation (ChIP) is a plus, so are skills in data mining and computational analysis.
Troubleshooting skills, independent analytical thinking and research planning skills.
Demonstrated ability to work independently and communicate with the laboratory staff and investigators.
Ability to maintain complete and organized records/reports.
Working knowledge of computer software and general lab equipment.
Base pay commensurate with experience.
All external candidates receiving an offer for employment will be required to submit to pre-employment screening for this position. Current employees applying for a new position within the university may be subject to this requirement. The screenings will include a criminal background check and, as applicable for the position, other background checks, drug screen, employment and education or licensure/certification verification, physical examination, certain vaccinations and/or governmental registry checks. All offers are contingent upon successful completion of required screening.
Please attach a copy of your most current signed performance evaluation (completed within the last 18 months) to your online account. If you have not received a performance evaluation, you may provide two current signed letters of recommendation (written within the last 18 months), preferably to include one letter from either a current or recent former supervisor. To attach these documents, go to: My Career Tools, Add Attachment, Attachment Type – Performance Reviews or Letters of Recommendation.
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