Environmental and Molecular Toxicology Assistant Specialist (Vogel Laboratory)
The position of Specialist has a narrow focus in a specialized area and provides technical or specialized expertise (e.g., with instrumentation and research equipment or with social science research methods) in the planning and execution of a research project. The Specialist stays apprised of emerging issues and problems and maintains technical competence in the designated area(s) to focus on the function of receptors for xenobiotics and inflammatory signaling to understand molecular mechanisms of the development of chronic inflammatory diseases promoted by exposure to environmental toxicant. Within this defined area and when appropriate, the Specialist is expected to provide leadership, facilitate teamwork and develop collaborative relationships with colleagues and to supply input into the planning of research and educational programs. Normally, Specialists do not have Principal Investigator (PI) status but may obtain permission by exception and/or collaborate with a PI in preparing research proposals for extramural funding. The Specialist is evaluated for merit and promotion using three basic criteria outlined below.
Major Responsibilities and Designated Areas of Expertise I. Research in Specialized Area (80% Effort) Under general supervision of the Principal Investigator, the Assistant Specialist will conduct research and analysis of the experimental data; 1) Analysis of molecular mechanisms causing immunotoxic effects as a result from exposure to environmental pollutants. 2) Identification of molecular markers (biomarkers) for risk assessment of the development of chronic diseases (autoimmune diseases, cancer) caused by exposure. 3) Development and application of in vitro models as alternatives to animal experiments. One major topic is the detection and assessment of the inflammatory and oxidative stress responses caused by chemicals from combustion processes. 3)Genotoxicity of fuel emission samples. The Single Cell Gel Electrophoresis assay (also known as Comet Assay) is a sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell will be employed. Human monocytic cells are used to evaluate the potential damage to DNA caused by exposure to fuel emission samples. The Assistant Specialist is responsible for conducting literature review, collecting and analyze data, constructing scientific graphs, documenting, publishing results, and collaborating with and mentoring junior researchers. Assistant Specialist collaborates with other research and extension personnel affiliated with research activities involving gaining a deeper understanding of the functional impact and molecular mechanisms of beneficial and detrimental ligands interacting with the AhR and cells of the innate immune system. Using both the vitro models and mouse in vivo models, to gain molecular insights into the development of chronic diseases mediated by environmental pollutants. These insights are invaluable to device strategies to program and reprogram cells toward a particular immune response allowing the development of new therapeutic substances and strategies. Preferably, the Assistant Specialist would be included in publications as evidence that work done by the Specialist significantly contributed to research findings and the publication of it.
II.Professional Competence and Activity (15%) Compile and document analysis and research findings for publications. qualifications include the participation in appropriate professional/technical societies or groups and other educational and research organizations. Candidate will review journal articles and discuss the significance of new techniques and other studies revealing similar results related to immuno-toxic effects and chronic diseases. Review research proposals, journal manuscripts, and publications related to area of expertise. Potential to present findings at lab meetings and/or conferences.
III. University and Public Service (5%) Effectively communicate with the principal investigator and project members. Mentor undergraduates and junior researchers working on complementary topics. Participate in project meetings and activities of committees and Centers within the Department and Campus providing input to the project.
BASIC QUALIFICATIONS: MS in toxicology, physiology, biochemistry, biology, ecology or in related fields or technical expertise equivalent to a masters degree in the area of specialization or five years of training in area of specialization. Experience in analyzing molecular mechanisms causing immunotoxic effects. Skills in isolation of proteins and nucleic acids including RNA and DNA is a requirement. Experience running PCR experiments. Experience using the Western Blot technique to identify protein expressions. Experience identifying molecular markers for risk assessments. Experience in development and application of in vitro models. Ability to process Single Cell Gel Electrophoresis assay (Comet Assay). Experience handling chemiluminescence instrument to run luciferase reporter assay. Experience with Genotyping of mice tissue samples. Skills to evaluate data from PCR analysis. 12.Ability to prepare graphs and tables to present data. Computers skills in MS Excel, Word and instrument specific programs required.
PREFERRED QUALIFICATIONS: Maintenance of cell cultures. Counting and seeding cells for culture plates. Preparation of histological tissue sections. Staining of tissue section with H&E and other dyes. Antibody staining and preparation of samples for Confocal microscopy. Skills for transformation and transfection experiments to run luciferase report assays.
TERM OF APPOINTMENT: This is a part-time (60%), one-year term appointment with possibility of renewal contingent on funding and performance.
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