We are seeking an experienced cell culture scientist who will perform research and development work at University of California San Francisco Parnassus Campus. The successful candidate will work under the direct supervision of Profs. Susan Fisher and Michael McMaster. We are seeking a scientist who will be part of a team deriving clinical-grade human embryonic stem cell lines in collaboration with a private-sector company. We are looking for an individual with extensive knowledge and experience in stem cell biology, cell banking, and cell biology techniques. Training will be provided as needed.
Required Qualifications • Junior rank- BA/BS degree with a major in biology or related science or four years of research experience • Assistant or Associate ranks- MS (or equivalent degree) with a major in biology or related science or five to ten years of research experience. • Full rank- A PhD (or equivalent degree) with a major in biology or at least 10 years of research experience • Two or more years of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position and demonstrating technical expertise in these areas; or an equivalent combination of education and experience. • Knowledge of cellular physiology as related to biochemical pathways, growth requirements for cells in vitro, and cellular responses to varying conditions (e.g., different oxygen tensions) • Knowledge of immunocytochemistry and other antibody-based techniques; light and fluorescence microscopy • Skills in various molecular biology methods such as RNA isolation and qRT-PCR analyses • Excellent knowledge of and proficiency in aseptic technique, analytical lab methodology • Knowledge of cell culture systems in which different substrates/media are employed • Computer proficiency including MS Office, Photoshop, photomicrography packages; ability to learn instrumentation software • Ability to work independently and in teams consisting of members with different degrees of knowledge or ability? • Good oral and written communication skills in English • Good organizational skills
Preferred Qualifications • MS/PhD • Two or more years of experience in working with human embryonic stem cells, including their maintenance, characterization and banking. • Knowledge of DNA/RNA purification, optimization of mammalian cell transfection, as well as PCR, qPCR, and immunoblotting/immunolocalization techniques • Proficiency and experience with human stem cell culture systems • Ability to perform FACS analyses • Experience in culturing/deriving new ESC lines
OBSTETRICS, GYNECOLOGY AND REPRODUCTIVE SCIENCES The Department of Obstetrics, Gynecology and Reproductive Sciences (Ob/Gyn & RS) is a major academic Department in the School of Medicine, engaged in clinical, research, and training activities at the Parnassus, Mt. Zion, Mission Bay and San Francisco General Hospital campuses, as well as satellite locations throughout the Bay Area, with an annual operating budget of $77.2 million. The Department has 86 full-time faculty, 136 other academic appointees, 18 post-doctoral fellows, 20 clinical fellows, 32 residents, 113 voluntary clinical faculty, and 310 staff. The mission of the UCSF Department of OBGYN & Reproductive Sciences is to improve the lives and health of all women through excellence, innovation and leadership in Patient Care, Scientific Discovery, Education, Advocacy. “Leading the way in women’s health.”
About UCSF The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.