Research - Laboratory/Non-Laboratory, Staff/Administrative
Research Technologist needed to manage daily operations of research laboratory investigating the dynamics of microbial pathogen exposures, immune responses, and human health.
Position will support research projects to investigate environmental and occupational exposures to microbial pathogens and human health outcomes. The primary focus of the lab is S. aureus, hepatitis E virus and influenza virus-related research including investigation of exposure and transmission routes, classification and molecular characterization of pathogens, and epidemiologic dynamics of immune responses and health outcomes. Work requires basic use of scientific methods, procedures and techniques; though training will be provided, previous experience and a profound understanding of theory behind experiments to perform data analysis and troubleshoot situations that need special attention with a substantial degree of independence are highly preferred.
Specific Duties & Responsibilities
Perform bacterial culture of environmental, human and animal specimens
- Testing of bacterial isolates for antimicrobial susceptibility (disk diffusion)
- Perform nucleic acid extraction (manual and automated)
- Perform RT-qPCR and endpoint PCR including polyacrylamide and agarose gel electrophoresis
- Prepare and test specimens for antibodies against pathogens and for pathogens (ELISA; Luminex)
- Maintain detailed records of quality assurance and quality control for specimens and specimen analyses;
- Record and analyze results, organize data, perform basic computations, and develop reports and summaries of analyses;
- Operate basic and advanced laboratory equipment, including automated nucleic acid extraction system, RT-qPCR instrument, microplate washer and reader, incubator, spectrophotometer, laminar flow hood, thermal cycler and other basic lab equipment.
Other responsibilities include:
- Contribute to general maintenance of the laboratory, equipment and supplies;
- Maintain and make minor adjustments to equipment, e.g. calibrations;
- Monitor inventory levels, order materials and supplies in accordance with established policies and maintain records.
Skills required: bacterial culture, isolation of bacteria and viruses, nucleic acid extraction, real time PCR, ELISA, Luminex multiplex immunoassays, cloning.
Minimum Qualifications (Required)
Bachelor's degree in biology, chemistry or related field.
Some related post-undergraduate work experience preferred.
Bachelor's degree in biology, chemistry, molecular biology, virology, microbiology, immunology or related field.
Experience in culture-based and molecular microbiology and immunology.
Experience in library prep for next generation sequencing and sequence analysis is preferred.
Special Knowledge, Skills, and Abilities:
Bacterial culture, isolation of bacteria and viruses, nucleic acid extraction, real time PCR, ELISA, Luminex multiplex immunoassays, cloning.
Equipment to be used: Nucleic acid extraction & qPCR instrument, Luminex instrument, ELISA reader.
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO37.5/02/CC Starting Hourly Pay Rate Range: $14.14 - $19.44/ Commensurate with Experience Employee group: Full Time Schedule: Monday - Friday; 8:30a-5:00p / 37.5 hours per week Exempt Status: Non-Exempt Location: 05-MD:School of Public Health Department name: 10001082-Environmental Health and Engineering Personnel area: School of Public Health
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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