Research - Laboratory/Non-Laboratory, Research Support - Laboratory/Non-Laboratory, Staff/Administrative
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Posted Job Title
Research Technician C
Job Profile Title
Technician, Research Laboratory C
Job Description Summary
The focus of the research is to understand how cyclical intermittent hypoxia affects lung cancer progression in mouse models of the disease. This individual will assist with breeding and genotyping mice, injecting mice with virus that turns on cancer or cloned cancer cells to turn on cancer, place them into cyclical intermittent hypoxia chamber and perform daily checks on the mice, harvest mice based on endpoints predetermined by the PI, perform all steps to preserve tissue with formaldehyde, cryopreserve and store at -80, slice tissue and obtain signals using immunohistochemistry, perform all steps to prep tissue or RNA/DNA/PCR/RNAseq work, perform all step for single cell prep and flow cytometry work, perform all steps for injecting mice with chemotherapy and induce an inflammatory response with LPS, harvest using a broncho-alveolar lavage, perform microCT scans on mice to assess tumor progression, use ITKsnap to segment the lung tumors, perform tests to assess cancer fatigue.
The person will be trained superbly in each of these techniques systematically. The highly motivated individual will earn authorship on research publications and present data at research meetings.
This position has an end date of 1 year with the possibility of extension and is contingent upon funding.
Manage mice exposed to cyclical intermittent hypoxia, troubleshoot all issues with cyclical intermittent hypoxia and gasses, perform husbandry including breeding, ear tagging and tail clipping and separate mice, arrange mice into cages based on age/sex for experiments (25%)
Wet bench experiments-make all solutions, purify DNA/RNA for PCR/RNAseq, run genotyping assays, prepare lung/heart, liver, kidney/adrenal glands, brain and spine tissue to then perform immunohistochemistry, perform single cell prep for flow, cell culture work for clone cells (25%)
Perform microCT scans in mice with lung cancer and segment tumors using ITKsnap (25%)
Perform injections of virus/clone cells to turn on cancer, weekly eye/tail/IP injections of chemotherapy, tracheal aspirations of LPS, broncho-alveolar lavage, harvest tissue based on experiments (15%)
Log everything online, maintain all logs of surgeries and narcotics, assist with data cleaning/analyses (5%)
Other duties as assigned (5%)
This position has an end date of 1 year with the possibility of extension and is contingent on funding.
Qualifications Bachelor's Degree Required, with 0-1 year of related experience or equivalent combination of education and experience.
Typically sitting at a desk or table; Occasional lifting 25 lb.
Job Location - City, State
Department / School
Perelman School of Medicine
$16.23 - $29.21
Affirmative Action Statement Penn adheres to a policy that prohibits discrimination on the basis of race, color, sex, sexual orientation, gender identity, religion, creed, national or ethnic origin, citizenship status, age, disability, veteran status, or any other legally protected class.
Special Requirements Background check required after a conditional job offer is made. Consideration of the background check will be tailored to the requirements of the job.
Internal Number: JR00016602
About University of Pennsylvania
Penn has a long and proud tradition of intellectual rigor and pursuit of innovative knowledge, begun by Benjamin Franklin in 1740. That tradition lives today through the creativity, entrepreneurship, and engagement of our faculty, students, and staff. Academic life at Penn is unparalleled, with an undergraduate student body of 10,000 from every U.S. state and around the world. The entering class of 2015 is the most talented and diverse in Penn's history. Consistently ranked among the top 10 universities in the country, Penn welcomes an additional 10,000 students to our top-ranked graduate and professional schools.