New Senior Research Specialist position in the Vanderbilt Center for Stem Cell Biology (VCSCB) at Vanderbilt University. The individual who fills this position will be a key team member in the Vanderbilt Genome Editing Resource (VGER), a Vanderbilt core facility and critical component of the VCSCB. The Senior Research Specialist will report to the Manager of VGER, and will play an essential role in the operations of both the VCSCB and VGER by developing and providing services to Vanderbilt investigators who need to derive new human pluripotent stem cell lines, and by performing gene editing in already established lines. VGER, by competently providing these services, enables Vanderbilt investigators to perform state-of-the-art cellular, molecular and/or developmental biology experimentation in pluripotent stem cells.
The Senior Research Specialist will work together with VGER staff to culture and maintain induced pluripotent stem cells, develop new pluripotent stem cell lines, perform genome editing in both cell lines and mice, analyze experimental results and help to write project summaries. In addition, the Senior Research Specialist will assist the Director and Senior Staff Scientist with design of new genome editing projects, and will contribute to the development of new services that utilize site-specific recombinases, transposases and other state-of-the-art genome manipulating techniques. The Senior Research Specialist will ensure the laboratory is prepared for experiments, write progress reports, and prepare and present data. By successfully helping investigators to produce and genetically-modify human pluripotent stem cell lines, the performance of the Senior Research Specialist will be critical to the missions of Vanderbilt, the VCSCB, and VGER. To achieve these goals a high level of communication, teamwork, organization, and performance is required.
The Vanderbilt Center for Stem Cell Biology (VCSCB) reports to the Dean of Basic Sciences in the School of Medicine. This unit facilitates scientific communication between faculty members and oversees the operations of two fee-for-service based core facilities, the Vanderbilt Genome Editing Resource (VGER) and Creative Data Solutions (CDS). VGER currently supports over one hundred laboratories at Vanderbilt in the production and use of genetically-altered mice, and seeks to expand its already substantial scientific impact through the development and performance of services related to the production and genetic modification of human pluripotent stem cells.
Duties and Responsibilities:
Work in cooperation with others to derive, maintain and validate new induced pluripotent stem cell lines
Implement new research projects and support the performance of ongoing research projects
Develop and implement protocols to efficiently edit the genome of induced pluripotent stem cells
Bring a team-oriented approach to the development of new methods and protocols
Perform CRISPR/Cas-mediated mutagenesis in mice, and assist with the analysis of experimental results
Work with others to assure the timely performance of genome editing experiments on a fee-forservice basis
Summarize and present experimental results in a clear, organized and coherent manner
Performs literature searches and make recommendations for research projects and protocols
Profile of an Ideal Candidate:
Bachelor's degree in biology or a related field is necessary.
Master's degree or PhD in biology or a related field is preferred.
Experience in Microsoft Office including Word, Excel, and PowerPoint is necessary.
Experience in the use of FileMaker Pro databases and other online data resources is preferred.
Experience performing CRISPR/Cas-mutagenesis, RNA expression analysis, and induced pluripotent stem cell derivation is preferred.
At least five years of laboratory experience is strongly preferred.
Previous cell culture experience is strongly preferred.
Basic knowledge of mouse genetics and husbandry is necessary.
Molecular biology skills including tissue culture, PCR, generation and purification of DNA is necessary.
Knowledge of mammalian development, gene regulation, cell differentiation and genome editing is strongly preferred.
Commitment to Equity, Diversity and Inclusion
Vanderbilt University is committed to achieving the goal of a diverse and inclusive academic community of faculty, staff, and students. We seek individuals who are committed to this goal and our campus values.
Internal Number: 2000064
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.