Research Specialist – Nachury Lab University of California, San Francisco Department of Ophthalmology
The Nachury Laboratory http://nachurylab.ucsf.edu within the Department of Ophthalmology at the UCSF School of Medicine studies the primary cilium, an organelle with central roles in developmental signaling pathways and sensory signaling.
We seek to hire one or more Research Specialists at the Junior, Assistant, or Associate level to study the basic biology of cilia using a combination of biochemistry, biophysics and live imaging. The Nachury Lab research team is broadly interested in the trafficking of signaling receptors in and out of cilia. Projects span biological scales from atomic-level structural biology to single-molecule tracking in live cells with occasional forays into animal studies. Dysfunction of the primary cilium causes a host of diseases (the ciliopathies) characterized by retinal degeneration, obesity, kidney malformations and skeletal anomalies. Part of our research is dedicated to understanding the mechanisms that underlie Bardet-Biedl Syndrome, a prototypical ciliopathy.
Department Overview: The UCSF Department of Ophthalmology is one of the leading ophthalmology departments in clinical care, training, and research nationwide. The Department of Ophthalmology is one of the largest recipients of federal grant funds among peer departments and has been in the top 10 in the National Institutes of Health (NIH) funding, currently ranking #2. Additionally, we have been the #1 recipient of the Research to Prevent Blindness funding nationwide. Overall, the Department has a wide-¬ranging portfolio of 60 active research grants. Research, patient care, teaching, and public service activities are at multiple UCSF campuses: Parnassus, Mt. Zion, Veteran’s Administration Medical Center (VAMC), Zuckerberg San Francisco General (ZSFG), and Benioff Children’s Hospital at Mission Bay. The Department occupies over 60,000 of assignable square feet of space and supports 25 full-¬time clinical faculty, 10 basic scientists, over 50 volunteer and visiting faculty, 12 postdoctoral scholars, 15 residents, seven clinical fellows, graduate students, visiting researchers, as well as clinic and research staff for a total of 235 full time employees (FTEs).
The successful candidates will:
Contribute technical assistance to students and postdoctoral fellows. Techniques may include but are not limited to: culture of mammalian cells, biochemical purification of protein complexes, purification of recombinant proteins, characterization of enzymatic activities, processing of cells for immunocytochemistry and fluorescence imaging of live and fixed cells.
Conduct experiments under the direct supervision of the Principal Investigator. The individual will have the opportunity to develop independence, acquire advanced technical and analytical skills and carry out his/her own research projects under supervision. In these roles, the Research Specialist is expected to assist in preparing figures for publications and grants and will gain authorship on publications.
Be responsible for maintaining mammalian cells and establishing stable cell lines with endogenous tagging using CRISPR/Cas9.
Be responsible for general lab organization including ordering supplies, organizing and maintaining inventories of stocks and reagents and adhering to safety regulations.
Specialists appointed at the junior rank must possess a baccalaureate degree in Biochemistry, Molecular Biology or related Biological Science degree, or must have at least four years of research experience.
Specialists appointed at the Assistant rank must possess a master’s degree (or equivalent degree) in Biochemistry, Molecular Biology, or related Biological Science degree, or must have at least 5 years of research experience.
Specialists appointed at the Associate rank must possess a master’s or terminal degree in Biochemistry, Molecular Biology, or related Biological Science degree and must have at least 5-10 years of research experience. Appointees at the Associate level should have significant record of academic accomplishment including published work, demonstrated record of University and/or public service. Additionally, appointees at the Associate level will provide considerable independence in planning and execution of research.
Efficient and organized with meticulous record keeping skills.
Highly motivated, ability to work well independently and in a team.
Excellent written and verbal communication skills.
At least 1 year of laboratory experience.
Familiarity with basic biochemistry (SDS-PAGE, western blot), basic molecular biology (primer design, PCR) and mammalian cell culture.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
Interested individuals should send their CV, Cover Letter, and the contact information for two reference at https://aprecruit.ucsf.edu/JPF02845. Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary and rank will be commensurate with the applicants experience and training.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.