Research - Laboratory/Non-Laboratory, Staff/Administrative
This position will remain open until filled.
UCSD Layoff from Career Appointment: Apply by 12/05/2019 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 12/16/2019. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Under supervision, the Lab Assistant 2 will manage the satellite mouse facility. Work with the Animal Care Program to coordinate mouse deliveries, housing and daily care. Manage mouse breeding colonies, including genotyping and mating. Manage AALAS compliance, personnel training, lab safety, lab equipment and supplies. Maintain laboratory infrastructure and supplies for the lab and attend lab meetings.
The incumbent will apply conventional methods, combining neuroanatomical, histological and behavioral approaches, and assist with experiments that aim to identify neural activity underlying behavior and learning. Under supervision by the PI and other senior lab personnel, conduct experimental procedures that include: performing surgeries on mice to prepare them for behavioral and imaging experiments; utilizing immunostaining and microscopy for histological experiments; and conducting behavioral experiments with head-fixed mice. Collect and organize data for record-keeping and publication.
Must be willing and able to work with rodents as animal subjects.
Must be able to work occasional evenings and weekends as required to keep steady workflow and meet deadlines.
Graduation from high school or a GED and one (1) year of laboratory experience; OR two (2) years of college including courses in the biological sciences; OR an equivalent combination of education and experience.
Relevant experience working with mice in laboratory setting. Must be proficient in handling them. Experience in behavioral studies with rodents preferred.
Demonstrated experience in managing mouse colonies, including genotyping, crossing and creating and maintaining accurate breeding records.
Knowledge of animal protocols and experience with animal care procedures, husbandry, anesthesia/analgesia requirements, postoperative care, euthanasia, experimental use records and carcass disposal.
Practical experience in small rodent surgery including anesthesiology procedures and chemical injections and knowledge for fine dissection.
Strong experience and ability to perform brain surgeries, including precise and stereotaxic injections of dyes and viruses, and use of different brain atlases for precise injections of tracer compounds.
Strong skills in histology, including perfusion, brain sectioning, immunostaining, routine neurohistology staining (e.g. Nissl and myelin), counterstaining including Giemsa and toluidin blue, labeling enhancement (gold, silver, nickel) and imaging (both epi and confocal).
Demonstrated experience in stoichiometry and chemistry for preparation of histological reagents, immunocytochemistry, and laboratory stock solutions.
Demonstrated knowledge of necessary care and precautions for use and storage of antisera, reagents, and for handling of hazardous and carcinogenic chemicals.
Proven experience in applying and adapting protocols for histological immunohistochemistry, including: use of peroxidase and avidinbiotin complexes and fluorochrome-conjugated antisera for single and multiple labeling.
Thorough knowledge and understanding of and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Programs.
Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals and use of protective items. Ability to keep safety training current and consistent with research involvement.
Proficient computer skills in MS Word, Excel, PowerPoint, email etc. with the ability to independently learn new programs.
Excellent English verbal and written communications skills, to articulate information on one's position, plan of action, evaluation or interpretation in a professional, logical, clear, effective, concise, and courteous manner. Skill at negotiating with various personalities and developing an internal and external network of contacts.
Strong interpersonal skills including tact, diplomacy and flexibility. Ability and experience to work in a team framework with staff, faculty and students. Experience participating and contributing to discussions regarding procedures and goals of depa
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