The Research Assistant position provides support to the Biochemistry Department. The role will perform research and technical duties for the laboratory. The individual will work closely with graduate students, postdoctoral fellows, and laboratory manager in a dynamic laboratory environment. The ability to function independently, as well as part of a team, is critical. Excellent organizational and communications skills (both verbal and written) are essential, as is attention to detail.
The Vanderbilt Biochemistry research laboratory investigates the structural basis for recognition, biochemical function and biological specificity of proteins and nucleic acids. Specifically, we study systems involved in bacterial pathogenesis, cell signaling, and cancer at the molecular level. Rather than focusing on specific techniques, we take a problem-oriented approach and use whichever techniques are necessary to answer the question at hand. Often, this requires complementary application of structural approaches including spectroscopy, scattering, crystallography, and microscopy.
The work unit is made up of faculty, research staff, postdoctoral fellows, and graduate students.
Duties and Responsibilities
Assists in Research and Development:
Researches and collects data using appropriate lab experiments, procedures, and techniques
Primary tasks involve sub-cloning, mutagenesis, and production of DNA vectors for protein expression
Large-scale bacterial over expression and purification of proteins using FPLC and HPLC
Writes and updates laboratory protocols
Modifies and plans research experiments, procedures, and techniques in optimizing research design
Synthesizes, analyzes and interprets data with minimal help
Reports on status of research activities in oral and written formats
Assembles and compiles research data for presentation and/or reports
Conducts lab space maintenance
Sets up, operates, and maintains laboratory equipment including, but not limited to - centrifuges, incubated shakers, FPLC, etc
Proper storage and maintenance of protein stocks
Assists with orders and maintenance of inventory and supplies
Performs Administrative Duties:
Oversees and trains research and support staff on equipment operations, technical procedures, and lab maintenance
Participate in weekly and bimonthly meetings to discuss project progress
Other tasks as directed by supervisor
Profile of an Ideal Candidate
A Bachelor's degree in biochemistry, biological sciences, chemistry, or related field from an accredited institution of higher education is necessary
A Master's degree in biochemistry, biological sciences, chemistry, or related field from an accredited institution of higher education is preferred
Experience in a structural biology laboratory is preferred
Knowledge and experience with microbiology, bacterial protein expression and purification ispreferred
Knowledge of chromatography instrumentation is preferred
Knowledge and experience with PCR and cloning is preferred
Knowledge and experience with mass spectrometry and ligation independent cloning techniques is preferred
Experience with organization, inventory, and record keeping is preferred
Commitment to Equity, Diversity and Inclusion
Vanderbilt University is committed to achieving the goal of a diverse and inclusive academic community of faculty, staff, and students. We seek individuals who are committed to this goal and our campus values.
Internal Number: 1902237
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.