Research - Laboratory/Non-Laboratory, Staff/Administrative
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The Division of Allergy and Infectious Diseases in the Department of Medicine has an outstanding opportunity for a full-time Research Technologist 2.
The technician will conduct microbiologic laboratory experiments using anaerobic and aerobic systems, and conduct experiments using human organoids. In this position, the technician will also perform nucleic acid extraction and purification, and quality analysis from human samples. The technician will also have the option of analyzing high-dimensional datasets generated in the context of an microbiome laboratory, if they have the appropriate computational skills.
The lab is located at the UW Medicine campus in South Lake Union’s “biotechnology hub” and focuses on understanding the impact of the gut microbiome on Vibrio cholerae infection and immunity to V. cholerae. Our laboratory has established that the gut microbiome impacts susceptibility to infection and influences immune responses to oral cholera vaccines. Although cholera is rarely seen in the US, this is a critical disease in global health settings and is especially important in children, where vaccines are not very effective. The WHO has set a goal of 90% elimination of cholera by 2030. To achieve this goal, our lab seeks to harness the gut microbiome to improve immune responses to vaccination and reduce susceptibility to disease. The lab has active collaborations with several global health focused laboratories and research groups in Bangladesh, Haiti, Canada and within the US. The position is anticipated to last at least two years.
This position requires prior laboratory experience, specifically in microbiology and preferably also in molecular biology. Experience in anaerobic microbiology is not required and the applicant will be trained in this area. The position has a high degree of autonomy and requires personal initiative, discretion and flexibility to perform experiments. All applicants should be highly organized and this position requires critical attention to detail.
Research -Culturing microbiota (gut bacterial species) from human samples using selective media and anaerobic conditions, using appropriate literature and product review for designing selective culture -Extraction of RNA and DNA from human samples, nucleic acid purification and evaluate nucleic acid quality -Conduct cell culture experiments including using human organoids -Perform immunological assays such as ELISA -Conduct qPCR for detection of virulence factor transcription in culture of Vibrio cholerae and assemble results -Follow strict methodology to execute experiments and analysis -May align and process raw sequence data -May analyze high-throughput sequencing data using existing R packages -Work directly with bench scientists and researchers to explain and modify experiments and routine analytic processes to best serve the needs of evolving projects.
Sample and equipment management -Ensuring maintenance and organization of laboratory equipment -Conducting sample inventory and organization as needed for ongoing projects -General laboratory maintenance and upkeep
Publications, presentations, and data requests -Create text, tables, figures, and charts for presentations and publications based on the data generated. -Provide referencing and other support for publications and presentations.
As a UW employee, you will enjoy generous benefits and work/life programs. For detailed information on Benefits for this position, click here.
Bachelor's Degree in an appropriate field of technology or science, PLUS one year of research experience equivalent to Research Technologist I OR one year of full-time post-baccalaureate college in an appropriate field of science or technology. Professional level experience may be substituted, year-for-year, for the formal academic degree.
Bachelor's degree in biology, molecular biology, microbiology, or a related field and 2-3 years of experience. AND:
Interest in global health research
Ability to resolve technical problems and use analytic skills
Ability to work independently and as a team member
Ability to maintain a detailed laboratory and well organized record of research performed and results obtained
Demonstrated organizational skills, self-motivation, flexibility, and strong communication skills.
Experience in molecular methods and microbiology
CONDITIONS OF EMPLOYMENT
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