Running adhesion assays (Microfluidics) on samples from patients, using endothelial cells, biological materials, under ambient or hypoxic conditions.
performing ELISAs of plasma proteins
purifying plasma factors
purifying membrane proteins from maintained cell lines
some molecular biology work
Collecting patient blood or tissue samples from clinic, hospital or CTRC.
For all work in the lab, the employee will be expected to use safe techniques in handling human blood including proper disposal and other safety measures as mandated by OSHA regulations. In running coagulation assays, the employee will be expected to draw blood and to prepare samples for the use in the appropriate instrument The employee will be expected to analyze data as it is being generated on the instrument and alert a senior researcher if data appears outside of established parameters so that samples can be rerun immediately if needed. The employee will be expected to recognize when a purification step has not yielded appropriate results and report to a senior researcher. For molecular biology, the employee will be expected to follow established procedures very closely and report results at each step.
Data analysis: The employee will be expected to understand when the results from an experiment are outside of the established parameters and to report to a senior investigator (as described above for specific procedures). The employee will be expected to organize their data clearly for interpretation by a senior researcher. The employee will not be asked to prepare talks or papers outside of the group setting although he/she will be asked to gather and collate data on his/her work for inclusion into presentations and to present his/her work within the context of the laboratory group’s weekly meeting.
Routine laboratory maintenance: For lab ordering, the employee will be expected to maintain a list of common reagents in an existing database and know their rate of use so as to maintain continuous supplies of the reagents. The employee will be expected to know the ordering procedures used in the laboratory and to coordinate with the appropriate administrative personnel.
The employee will be assisting other laboratory personnel in disposing of waste according to OSHA guidelines. In addition, the employee will be asked to do some tissue culture work to maintain existing cell lines. In this work, the employee will be expected to use sterile techniques to maintain sensitive cell lines. The employee will be expected to note cell growth and health outside of normal parameters and report any abnormalities.
Education and Experience:
Demonstrated possession of the competencies necessary to perform the work.
Previous experience in a research laboratory. Knowledge of physical and chemical properties of RNA, DNA and proteins. Knowledge of tissue culture and molecular biological techniques. General knowledge of laboratory techniques, the use of laboratory equipment and basic laboratory procedures. Ability to make independent judgments. Ability to understand and follow complex written and oral instructions as they relate to the laboratory analysis. Ability to document assay results, prepare written reports and enter data in computer. Good communication skills.
Strongly prefer candidates who have a Bachelor’s degree in Biological Sciences. Competency with flow cytometry.
The University of North Carolina at Chapel Hill is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender, gender expression, gender identity, genetic information, race, national origin, religion, sex, sexual orientation, or status as a protected veteran.
Internal Number: 170608
About The University of North Carolina at Chapel Hill
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